EVOS M3000 Imaging System

Objectively precise, incredibly easy

Contact your local representative for availability of AMF3000.

The EVOS M3000 Imaging system was designed to be the workhorse of any cell culture or research laboratory by democratizing the ability to produce reliable, stunning high-quality cell images and video. This system automatically and precisely measures cell confluency in real time, with no expertise required. This compact, upgradeable, easy-to-use system provides two-color fluorescence, color-imaging, brightfield, and phase contrast options, making it your go-to manual microscope.

Features

Cell confluency in seconds

Built-in automated real-time image analysis for measuring cell confluency

Versatile

Capture images in brightfield, phase contrast, color imaging, and fluorescence

Flexible

Compatible with the full selection of EVOS objectives (1.25-60X) and light cubes

No training required

Simple user interface with touchscreen display

Space-saving design

Our smallest EVOS footprint fits easily on the lab bench or in a cell culture hood

Connectivity

Network-capable instrument facilitates seamless data transfer, storage and collaboration enhancing productivity and data management

EVOS real-time confluency tool

The patent-pending EVOS real-time confluency tool can typically measure confluency in less than 1 second without requiring any image capture, making it excellent for eliminating user bias in routine cell culture. The output from this proprietary bioapplication correlates well with confluency measurements from other software, e.g., Cell Profiler, and is standard on all M3000 instruments.

Induced pluripotent stem cells imaged over time with the EVOS M3000 instrument. Human fibroblast-derived induced pluripotent stem cells were cultured on a vitronectin-coated 6-well plate in Gibco Essential 8 Flex Medium for 3 days. Cells were imaged with the EVOS M3000 instrument under phase microscopy with and without the automatically generated confluency mask and measurement.

Specifications

The EVOS M3000 Imaging System is a digital inverted microscope for 2-color fluorescence, transmitted light, and color imaging. Download Technical Specifications Sheet

Attribute		Description
Optics	Imaging modes	Fluorescence, brightfield, color brightfield, and phase contrast
	Imaging methods	Single color, multicolor, and movie capture
	Optical system	Infinity‐corrected optical system; RMS‐threaded objectives with 45 mm parfocal distance
	Illumination	Adjustable intensity LED (>50,000-hour life per light cube)
	Contrast methods	Epifluorescence and transmitted light (for brightfield and phase contrast applications)
	Light cube capacity	2-position chamber for up to 2 fluorescent LED cubes
	Light cubes	Selection from Invitrogen EVOS fluorescent LED cubes, including, but not limited to: • DAPI (357/447 nm) • GFP (482/524 nm) • RFP (542/593 nm) • Texas Red (585/628 nm) • Cy5 (635/692 nm) Motorized LED cube interchange mechanism. Custom LED cubes available on request
	Objective capacity	4-position manual turret (not automated)
	Objectives	Selection from more than 35 high-quality, long working distance (LWD) and coverslip-corrected (CC) objectives; magnification from 1.25x to 60x
	Condenser	4-position turret with a clear aperture and 3 phase annuli
	Focus mechanism	Manual and touchscreen driven focus with sub-micron (0.15 μm) resolution
	Camera	High-sensitivity color CMOS camera (2064 × 1536-pixel resolution, 3.2 Megapixels) with 3.45‑µm pixel resolution
	Captured images	8-bit monochrome: TIFF, PNG, JPG 8-bit color: TIFF, PNG, JPG Movies: MP4
	LCD display	10.1-inch high-resolution LCD touchscreen display (1920 × 1200-pixel resolution)
Mechanics	Stage control	Manual (optional mechanical stage add-on)
	Stage size	7 x 9 in (18 x 22.2 cm)
	Mechanical stage (optional) range	5 x 3 in (12 x 8 cm)
Vessels	Compatibility	Microscope and chamber slides Hemocytometers 6-, 12-, 24-, 48-, 96-, and 384-well microplates 35-, 50-, 60-, and 100-mm petri dishes T-25, T-75, and T-175 flasks
Software and PC	Integrated onboard operating software	Touchscreen controlled embedded graphical user interface (eGUI) Confluence measurement with motion detection and sensitivity adjustment over live/captured image Contrast stretching over live/captured image Single capture and video recording
	Image saving	Save images on embedded hard drive, an external USB device, a local network drive, Thermo Fisher Connect, or Microsoft OneDrive
	Computer	Embedded PC with 8 GB RAM
	Storage	120 GB SSD, support for USB 3.0 memory stick, network drive and cloud storage
System	Output ports	1 USB 3.0, 2 USB 2.0
	Networking capability	Connection through Windows/SMB network wirelessly using the supplied Wi-Fi adaptor
	Cloud connectivity	Connect to the Thermo Fisher Connect Platform or Microsoft OneDrive for remote access to images and data via the internet
	Power supply	Universal power supply (12 V, 5 A) and power cord
Physical characteristics	Dimensions (L x W x H)	19.1 x 11.7 x 12.7 in. (48.6 x 29.6 x 32.3 cm)
Physical characteristics	Weight	18.4 lb (8.3 kg)

Powerful, intuitive software

Users can easily share files from the instrument via the Thermo Fisher Connect cloud ecosystem, as well as MS OneDrive, making it an exceptional solution for research teams.

Real-time image analysis for cell confluency allows rapid confluency measurements to be performed across multiple points in a sample, as well as between samples, without needing to capture and analyze still images.

As with every EVOS instrument, overlay scale bars, gridlines, or adjust brightness, saturation, and gamma correction prior to imaging.

Following acquisition, scroll through all images and video in a gallery view to tag those of interest or easily delete any you don’t want.

Powerful onboard software with image processing tools built in. The user-friendly EVOS M3000 interface allows for effortless image capture in transmitted light or fluorescence mode (left), while also facilitating the exploration of saved images through the image gallery feature (right).