Selection guide for human T cell isolation

Which human T cell isolation kit is right for your application and sample type? Dynabeads has kits optimized for specific sample types broadly for whole blood, mononuclear cells (MNC) and bone marrow, or for those focused directly on MNC.

 Highest purity for molecular assays Affordable & pure, flow-compatible cells Extreme purity & physiological quality Untouched—most physiological relevant cells
    
Isolation methodDepletion or positive isolation Depletion or positive isolation Positive isolation Negative isolation
Starting samplesAny sample (whole blood, MNC, bone marrow) Any sample (whole blood, MNC, bone marrow) Any sample (whole blood, MNC, bone marrow) MNC
# MNC processed (mL whole blood processed)1-2 x 109 MNC
200 mL whole blood		 1-2 x 109 MNC
200 mL whole blood		 2 x 109 MNC
80 mL whole blood		 1 x 109 cells
PurityHighest
98-100%		 Highest
98-100%		 Higher
95-99%		 High
90-95%
YieldHighest
95-100%		 Highest
95-100%		 High
70-85%		 Standard
50-70%
Viability>95% >95% >95% >95%
Isolation time~30 min ~30 min ~60 min ~50 min
Isolation temp.Cold Cold Cold Cold
ApplicationsDepletion of unwanted CD2+ T cells or positive isolation for molecular applications, e.g., isolation of proteins & nucleic acids Depletion of unwanted CD3+ T cells or positive isolation for molecular applications, e.g., isolation of proteins & nucleic acids Cellular assays (cell culture, cell activation/proliferation, cytokine studies)

Molecular isolation of proteins & nucleic acids		 Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free target cellsNo No Yes (but antibody present) Yes
Compatible with
flow cytometry/sorting		No No Yes Yes
Shelf life from production date3 years 3 years 2 years 2 years
Required materialsMagnets Magnets Magnets Magnets
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