Vanquish Method Development HPLC and UHPLC Systems

Automated and accelerated method development

Accelerate and automate your method development using Thermo Scientific Vanquish Method Development HPLC and UHPLC systems. These systems can optimize separation conditions by testing different combinations of buffers on up to six HPLC columns. Vanquish Method Development HPLC and UHPLC systems use Thermo Scientific Chromeleon Chromatography Data System (CDS), which features downloadable eWorkflows to simplify automated column and buffer scouting while enabling secure and central data storage. Achieve fully automated method development with ChromSwordAuto Chromeleon Connect and ChromSword AutoRobust Chromeleon Connect. Chromeleon CDS is also compatible with the S-Matrix^® Fusion QbD^® software package.

Highlights

Automated column and eluent screening option

Screen up to 13 eluents by equipping your Vanquish Method Development HPLC and UHPLC system with a solvent selection valve for extensive, automated screening. The Thermo Scientific Viper Method Scouting Kit contains all the tubing and near-zero dead volume connections to screen four columns automatically without the need for any user interaction.

Universal and sensitive detection techniques

Detect all non- or semi-volatile analytes using a near-universal Thermo Scientific Charged Aerosol Detector. Additionally, gain more confidence in identification using the Thermo Scientific ISQ EC and EM single quadrupole mass detectors for peak identification and impurity profiling.

Automated, software-assisted method development

Automate, simplify and accelerate method development. The Vanquish Method Development HPLC and UHPLC systems are controlled by Chromeleon CDS and compatible with leading method development software packages, such as ChromSwordAuto Chromeleon Connect or Fusion QbD software.

Secure data storage

The Chromeleon Data Vault ensures secure data storage and accessibility for users within the CDS infrastructure. With Vanquish Method Development HPLC and UHPLC systems, all data generated is safely backed up in Chromeleon CDS Data Vault, enabling easy data exchange throughout the organization.

Optimized for small and large molecules
When built using Vanquish Flex and Vanquish Horizon UHPLC components, these systems are biocompatible, enabling robust performance, even with high-salt gradients, while minimizing unwanted interactions between the analyte and system components. ChromSwordAuto Chromeleon Connect includes dedicated algorithms for more efficient separations of large biomolecules.

Method validation
Automate design and execution of robustness testing using ChromSword AutoRobust Chromeleon Connect and accelerate your method validation using the workflows available in the Chromeleon 7 Extension Pack ICH templates.

For more information, download the Vanquish Method Development LC system product spotlight

What are the stages of HPLC method development?

Method scouting includes screening column and mobile phase conditions to choose the most promising combination for a successful HPLC separation. Traditionally, method scouting requires a significant amount of effort for manual column and mobile phase switching as well as instrument method creation. This process can be automated and streamlined using Viper fingertight fittings and solvent extension kits, and software packages such as ChromSwordAuto Chromeleon Connect and Fusion QbD.
Method optimization, involving iterative testing of various separation conditions of the HPLC method, is performed to achieve the best possible resolution, speed, etc. Operators must run many samples and repeatedly make decisions based on results. This is the most time-consuming stage of method development and requires a great deal of user experience and knowledge. ChromSwordAuto Chromeleon Connect and Fusion QbD software significantly accelerates this step by rapidly analyzing separation data and automatically optimizing the HPLC method.
Robustness testing is required to determine the impact of parameter variation on separation quality and is crucial for method validation. DOE and robustness testing can be automated using ChromSword AutoRobust Chromeleon Connect or Fusion QbD software.
Method validation is the industry-specific process for determining whether a method is fit for purpose. Secure documentation of the method development process is necessary in a variety of industries. Chromeleon CDS ICH validation templates and documentation of all stages of the workflow can be stored in the secure Chromeleon Data Vault to ensure compliance.

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System specifications

The table below provides an overview of the system specifications for the Vanquish Method Development HPLC and UHPLC systems for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category	Vanquish Method Development HPLC and UHPLC Systems
System pressure, maximum	700, 1000 or 1500 bar, depends on system configuration
Flow range	0-5, 0-8 or 0-10 mL/min, depends on pumps selected
Pump options (default delay volume)	Binary (35, 200 or 400 µL) Quaternary (679 µL) Dual (679 µL) Isocratic Delay volume depends on pump(s) selected
Biocompatible	Yes, depends on system configuration
pH range	2-12, chloride concentration up to 1 mol/L or 1-13 (chloride concentration up to 0.1 mol/L), depends on system configuration
System design	Modular
Touchscreen user interface display compatible (optional)	Yes, depends on system configuration
Solvent monitoring system compatible (optional)	Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer	Yes, depends on system configuration
SmartInject technology for maximum injection precision and improved column lifetime	Yes
Injection volume range	Standard: 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon) Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core) Depends on sampler selected
Sample cooling	Yes, 4-40°C Depends on sampler selected
Sample capacity (standard) Based on standard 12 mm OD vials (≤ 1.5 mL)	4 racks of 54 vials (≤ 1.5mL) each, other rack formats available + capacity of 12 x 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available	Yes, with Vanquish Charger
Column compartment thermostatting	Yes, 5-120°C, depends on column compartment selected Still air and forced air
Column compartment pre-heater	Active and passive Depends on system configuration
Column compartment post-column cooler	Yes Depends on column compartment selected
Detector choices	Vanquish Charged Aerosol Detector Vanquish Diode array Detector Vanquish Fluorescence Detector Vanquish Multiple Wavelength Detector Vanquish Variable Wavelength Detector Refractive Index Detector Mass Spectrometer

Component specifications

Standard Vanquish Method Development HPLC and UHPLC systems are made up of the following components. Download the associated specifications sheet for more detail on each component.

Vanquish Horizon Method Development UHPLC System

Vanquish Flex Method Development UHPLC System

Vanquish Core Method Development HPLC System

Pump

Download Specification Sheet

Vanquish Binary Pump H

Vanquish Binary Pump F
or
Vanquish Quaternary Pump F
or

Vanquish Dual Pump F

Vanquish Binary Pump C
or
Vanquish Quaternary Pump C

Autosampler

Download Specification Sheet

Vanquish Split Sampler HT or