Ribonucleases (RNases) | Thermo Fisher Scientific

Nucleases that catalyze the degradation of RNA, used to remove RNA from DNA solutions.

Ribonuclease	Applications	Reaction catalyzed	Substrate	Reaction products
RNase A, DNase and protease-free	Removal of RNA from plasmid or genomic DNA preparations Removal of RNA from recombinant protein preparations Ribonuclease protection assays	Cleavage of phosphodiester bonds between 3’-C or 3’-U residues and the 5’-OH residue of an adjacent nucleotide	ssRNA	3’-CMP 3’-UMP oligonucleotides with terminal 3’-CMP 3’-UMP
RNase T1	Removal of RNA from DNA solutions RNA sequencing Ribonuclease protection assays	Cleavage of phosphodiester bonds between 3’-G residues and the 5’-OH residue of an adjacent nucleotide	ssRNA	3’-GMP oligonucleotides with terminal 3’-GMP
RNase A/T1 Mix	Removal of RNA from DNA solutions Removal of RNA from recombinant protein preparations Ribonuclease protection assays	Combines the activities of RNase A and RNase T1	ssRNA	3’-CMP 3’-UMP 3’-GMP oligonucleotides with terminal 3’-CMP 3’-UMP or 3’-GMP
RNase I	Removal of RNA from DNA solutions Removal of RNA from recombinant protein preparations Ribonuclease protection assays	Cleavage of all phosphodiester bonds in an RNA template	ssRNA	3’-NMP
RNase H	Removal of mRNA prior to synthesis of second strand cDNA Removal of RNA after first strand cDNA synthesis Removal of the poly(A) sequences of mRNA after the hybridization with oligo(dt)	Cleavage of RNA phosphodiester bonds in RNA-DNA hybrids	RNA in RNA-DNA hybrids	5’-P oligonucleotides

ss – single-stranded
ds – double-stranded
NMP – ribonucleoside monophosphate