Dialyzed FBS is manufactured using a process that significantly reduces the concentrations of small (low molecular weight) molecules such as amino acids, hormones, and cytokines while avoiding the precipitation of serum proteins.
Dialyzed fetal bovine serum is recommended for special applications where the normal concentrations of small molecules in FBS may be of concern, for example, in studies requiring higher levels of control over certain small molecules in the cell culture environment.
Dialyzed FBS vs FBS
Whereas standard FBS will contain higher concentrations of small molecules (e.g., amino acids, hormones, cytokines), the careful filtration process used to produce dialyzed FBS greatly reduces the concentrations of these molecules, facilitating more precise control of nutrients and cell signaling molecules in the cell culture medium.
Why researchers choose dialyzed FBS
Watch to discover how the depletion of small molecules in dialyzed FBS allows researchers to control for molecules of interest in their cell culture experiments.
Features of Gibco dialyzed fetal bovine serum
- Dialyzed by tangential flow filtration (MW 10,000 cut-off)
- Dialyzed against 0.15 M NaCl until glucose <5 mg/dL
- Performance tested for cloning and plating efficiency
- Available in the innovatively designed, aliquot-free Gibco One Shot FBS 50 mL bottle
Research areas using dialyzed FBS*
- Proteomics (mass spectrometry)
- Mitochondrial function studies
- Glucose/amino acid deprivation studies
- Isotope labeling
- Cellular signaling and reporter assays
* These results are based on a review of approximately 10,000 publications using query terms based on the six specialty FBS products offered by Thermo Fisher Scientific. These terms were generated by the MeSH (medical subject headings) taxonomy based on the full text of the paper.
Order Gibco dialyzed FBS
- Tang R, Kimishima A, Ishida R, et al. (2020) Selective cytotoxicity of epidithiodiketopiperazine DC1149B, produced by marine-derived Trichoderma lixii on the cancer cells adapted to glucose starvation. J Nat Med 74(1):153–158.
- Hoshi T, Miyano SW, Watanabe H, et al. (2019) Lenvatinib induces death of hepatocellular carcinoma cells harboring an activated EGF signaling pathway signaling pathway through inhibition of FGFR-MAPK cascades. Biochem Biophys Res Commun 513(1):1–7.
- Moreno-Mendez E, Hernández-Vázquez A, Fernández-Mejía C. (2019) Effect of biotin supplementation on fatty acid metabolic pathways in 3T3-L1 adipocytes. Biofactors 45(2):259–270.
- Yenugonda V, Nomura N, Kouznetsova V, et al. (2017) A novel small molecule inhibitor of p32 mitochondrial protein overexpressed in glioma. J Transl Med 15(1):210.
- Mandal A, Das S, Kumar A, et al. (2017) L- Arginine Uptake by Cationic Amino Acid Transporter Promotes Intra-Macrophage Survival of Leishmania donovani by Enhancing Arginase-Mediated Polyamine Synthesis. Front Immunol 8:839.
Resources
Fetal Bovine Serum Basics
Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.
Cell Culture Basics
Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.
Gibco iMatch Sera Lot Matching Tool
Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.
Related products
Support
Find your regional sales representative
Connect with a regional FBS sales representative.
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support?
Contact our expert team for technical and application support of laboratory products.
For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.