Advances in both flow cytometry reagents and instrumentation allow researchers to run increasingly complex multicolor experiments. One of the biggest challenges in multiparameter flow cytometry is selecting the right combination of fluorophores and antibody conjugates so that the need for compensation and spillover adjustments is kept to a minimum while the quality and accuracy of the data are not compromised. In this section you will find educational resources and tools to help you design and execute multicolor flow cytometry experiments, including panel design tool, articles, application notes, on demand webinars, and more.
Panel design and multicolor flow cytometry features
Flow cytometry panel design tool
Flow Cytometry Panel Builder
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
BioProbes article
Flow cytometry panel design: The basics
This article provides an overview of panel design and what you need to know to get started.
Panel design and multicolor flow cytometry learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Scientific Poster (2017) | T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes | antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright |
| Scientific poster (2009) | A comparative study of 25 different CD4 conjugates | Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| Tutorial video | Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra | absorbance, emission, excitation, spectra |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Flow Cytometry | flow cytometer calibration, multicolor flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Fluorescence | flow cytometry, fluorescence imaging |
| Tutorial video | Molecular Probes Tutorial Series—Overview of Filters and Light Sources | emission, excitation, filters, light cubes, light sources |
| Webinar | Basics of flow cytometry, Part I: Gating and data analysis An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of multi color flow cytometry panel design With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
| antibodies, compensation, multicolor flow cytometry |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.