In this section you willfind educational resources such as application notes, webinars, videos, articles and more to assist in your cell proliferation experiments using flow cytometry.
Cell proliferation features
Guided learning
T cell stimulation and proliferation eLearning course
This modular, animated, and narrated eLearning course was developed to provide a succinct, contextual summary of T cell activation and the methods used to measure T cell function. There are knowledge checks throughout the course as well as a practical application session to test what you have learned.
BioProbes article
An evaluation of dyes for tracking cell proliferation by flow and image cytometry
This article reviews the different methods for tracking cell division using fluorescent dyes and flow cytometry. Additionally it presents the different experimental criteria for choosing a particular method or dye for cell proliferation analysis.
Cell proliferation learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Guided learning | T cell stimulation and proliferation eLearning course | antibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells |
| Protocols | BrdU staining protocol for flow cytometry (Invitrogen eBioscience reagents) | cell proliferation, flow cytometry |
| Protocols | CellTrace CFSE Cell Proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | CellTrace Far Red Cell Proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | CellTrace Violet Cell Proliferation Kit protocol | cell proliferation, flow cytometry |
| Protocols | Staining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents) | cell proliferation, flow cytometry |
| Scientific Poster (2016) | A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry | Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | cell proliferation, Click-iT, flow cytometry, immunophenotyping |
| Scientific Poster (2014) | Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing | Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry |
| Scientific Poster (2013) | Cells coming to life: Tools for visualizing immune response | endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2011) | The next step in the analysis of lymphocyte proliferation | Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents |
| Scientific poster (2010) | Analysis of proliferation dynamics in primary and immortalized human cells | Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2010) | Functional characterization of a novel fluorescent dye for proliferation analysis | Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents |
| Scientific poster (2010) | ;Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation | Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes |
| Scientific poster (2009) | Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometer | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2008) | Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method | Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2008) | Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry | Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation |
| Scientific poster (2008) | Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2006) | Novel violet-excited reagents for detection of viability and vitality | Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents |
| CYToMORE lecture video (2016) | Novel reagent solutions for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometry assays |
| Technology video | Click-iT Plus EdU Proliferation Assay Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies. | cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Webinar | An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |
| Webinar | DNA content cell cycle analysis using flow cytometry Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss: an overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content, selection of DNA dyes for live cell and fixed cell analysis and tips and tricks for consistent results. | cell counting, cell cycle, cell proliferation, flow cytometry |