Pluripotent stem cell passaging or dissociating can be accomplished using various methods depending on your culture medium and application. Whether you prefer to passage your cell enzymatically or manually, Gibco media has the solution for your PSC subculture.
Which passaging method is right for you?
| Order | Order | Order | Order | Order | Order | |
| Recommended for clump passaging in feeder and feeder-free cultures | Standard reagent for clump passaging in feeder and feeder-free cultures | Quick and gentle dissociation, recommended for single cell passaging | Recommended for clump passaging with feeder-free Essential 8 Medium | Optimal for clinical research applications that require human origin materials only | Quick and easy manual passaging tool for feeder and feeder-free cultures | |
| Product name | Dispase | Collagenase IV | Accutase | UltraPure 0.5M EDTA | TrypLE Select CTS | StemPro EZPassage Disposable Stem Cell Passaging Tool |
| Recommended applications | Passaging in clumps | Passaging in clumps | Dissociation to single cells | Passaging in clumps | Passaging in clumps or single cell passaging | Mechanical passaging in clumps |
| Source origin | Bacillus polymyxa | Clostridium histolyticum | Marine | Chemical animal-origin free | Recombinant enzyme animal-origin free | Not applicable |
| Form | Lyophilized powder | Lyophilized powder | Liquid, ready to use | Liquid, dilute 1,000x | Liquid, ready to use | Individually wrapped |
| Storage temperature | 2–8°C | 2–8°C | 2–8°C | Room temperature | Room temperature | Room temperature |
| Shelf life of liquid format | 2 weeks | 2 weeks | 2 years | 2 years | 2 years | Not applicable |
| Inactivation method | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Not applicable |
| Unit size | 5 g | 1 g | 100 mL | 4 x 100 mL | 100 mL | Box of 10 units |
| Optimized concentration for use | 2 mg/mL | 1 mg/mL for feeder-dependent, 10 mg/mL for feeder-free | 1X Solution | 0.5 mM | 1X Solution | Not applicable |
| Number of plates passaged/unit | 1,250 x 60-mm plates | 500 x 60-mm plates at 1 mg/mL, 50 x 60-mm plates at 10 mg/mL | 50 x 60-mm plates | 50,000 x 60-mm plates | 50 x 60-mm plates | 10 plates |