High Coverage Gene Expression Profiling (HiCEP)

Whole transcriptome expression profiling is historically performed using hybridization-based microarray methods. There are a number of limitations to microarray-based approaches such as low sensitivity and specificity, poor dynamic range, as well as a need for prior sequence information. The HiCEP method was developed to address the shortcomings in gene expression profiling and provide a sensitive method for detecting a large proportion of transcripts in both known and unknown genes, with a low false positive rate.