Our qIP Protein Interaction Kits use anti-HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and TurboLuc luciferase enzyme (Tluc) tagged protein pairs expressed in mammalian cells. The quantitative immunoprecipitation (qIP) system uses Tluc enzyme to accurately and precisely reflect the abundance of a specific co-IP product, to help avoid the need for time-consuming gel electrophoresis, western blotting, and band densitometry.
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Featured qIP products
An agarose bead format of our qIP kit, using HA tagging
Exploit the convenience of magnetic bead-mediated qIP
Recover and quantify proteins that bind to your Myc-tagged bait protein
Magnetic beads make quantitative immunoprecipitation fast and robust
Features of qIP kits
- Quantitative—integrated luciferase assay enables direct measurement of co-IP products, and control system helps ensure accuracy and normalization
- Sensitive—bright bioluminescence signal allows detection of weak interactions
- Time-saving—final read-out is relative luminescence units (RLU), rather than elution and western blotting
- Simple and fast—incubation and wash steps use spin columns or microcentrifuge tubes
- Robust—vectors, kit, and qIP method is compatible with various mammalian cell lines (293T, HEK293, NIH3T3, and CHO-K1)
- Versatile—highly customizable assay platform :
- Additional cloning vectors are available for N- and C-terminal fusions
- HA- and c-Myc tag kits are available in both agarose and magnetic bead formats
- Assay reagents and buffers are available separately
Schematic of the qIP assay procedure
Resources
For Research Use Only. Not for use in diagnostic procedures.