More Correct Calls With Higher Signal-to-Noise Ratios
Life Technologies BioPrime® labeling systems enable reliable and consistent genomic DNA labeling and efficient removal of free dye and nucleotides. And because results obtained using these reagents exhibit improved signal-to-background and reduced channel bias, you can count on greater accuracy.
| Sample Type | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Platform | gDNA | ChiP* | FFPE | DNA Input Amount | Labeling Method | Label | Master Mix | Product | |
| Non-Platform Specific |  | | 5 ng–3 μg | Direct | AlexaFluor® 3 AlexaFluor® 5 (included) | | BioPrime® Total Genomic Labeling System 18097011 - (30 reactions, w/ purification) 18097012 - (30 reactions, w/o purification) 18097010 - (10 reactions, w/ purification) | ||
| | 250 ng–1 μg (Bac arrays) 4 μg (cDNA arrays) | Direct | User’s choice (not included) | BioPrime® aCGH Genomic Labeling System 18095011 - (30 reactions, w/ purification) 18095012 - (30 reactions, w/o purification) | ||||
| Array-dependent | Direct | AlexaFluor® 3 AlexaFluor® 5 (included) | BioPrime® Total FFPE Genomic Labeling System A10965011 - (30 reactions, w/ purification) | |||||
| * ChIP must first be performed using a kit such as the MAGnify™ Chromatin Immunoprecipitation System, Cat. No. 492024 | |||||||||
Repetitive Sequence Blocking Reagents
| Product | Size | Cat. No. |
|---|---|---|
| Human Cot-1 DNA®-Fluorometric QC | 1 mg | 15729101 |
| Human Cot-1 DNA®-Fluorometric QC | 500 μg | 15279011 |
| Mouse Cot-1 DNA | 500 μg | 18440016 |
| Yeast tRNA | 25 mg | 15401011 |
Which BioPrime® system is right for me?
The BioPrime® Total aCGH reagents offer various configurations to allow for optimal performance on arrays based on premier enzymes, primers, dyes, and purification systems. For two-color array CGH and ChIP-on-chip experiments, we recommend the BioPrime® Total Genomic Labeling system. The BioPrime® Total Genomic Labeling system allows for the lowest starting input material, includes application-optimized dye-labeled nucleotides (with Alexa Fluor® 3 and Alexa Fluor® 5 dyes), and is the simplest, easiest-to-use kit in the product family. For researchers who wish to use other dyes, we offer BioPrime® aCGH Genomic Labeling systems without dyes—this allows you to take advantage of the higher-performance enzymes and reagents in our BioPrime® kits to deliver superior yield and better total dye incorporation.