The One Shot FBS: Fetal bovine serum in convenient 50 mL bottles
Gibco One Shot FBS is an aliquot-free solution for serum use, minimizing contamination and saving time. The One Shot FBS 50 mL bottle is designed to eliminate a tedious lab task and significantly improve your cell culture results.
Videos: Gibco One Shot FBS eliminates the need to aliquot FBS
Streamline your serum prep with One Shot FBS
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Researcher quote
“This bottle allows for convenience of speed while maintaining a sterile product. As we have lots of undergrads in and out of the lab, this ensures that one mishap with not-so-aged hands doesn't ruin a whole pack of FBS.”
Bethany C. Prudner, PhD, Metabolomic Sarcoma Lab, Washington University in St. Louis
Explore the many benefits of One Shot FBS
End the need to aliquot FBS
Aliquoting FBS into 50 mL conical tubes increases the chances of compromising your results. The One Shot FBS 50 mL bottle’s size and design eliminates your need to aliquot FBS, putting you in control of your time, contamination risk, and volume consistency.
Pour and pipet with ease
The bottle’s wide mouth and chamfered edge enable smooth, burp-free pouring. In addition, whether pouring or using a 25 or 50 mL pipette, you’ll be able to get every last drop.
Stop the freeze-thaw-repeat cycle
Skipping the FBS aliquot step means you only have to freeze then thaw your serum once—saving considerable time and effort. Because the bottles are engineered to withstand freezing, they won’t crack and pop their lids like conical tubes often do.
End labeling mishaps
Missing SKU or lot numbers, expiration dates, origin—or even the entire label—can cause a chain reaction of problems. The One Shot bottle has an easy-to-read package label, so you know exactly what you’re using.
Free up freezer space
The One Shot FBS package design is optimized to ease the burden of FBS storage on your freezer capacity, improving FBS storage in your lab. In fact, the One Shot FBS 10 x 50 mL carton allows you to store up 57 L more than our 100 mL bottle.
Pack the rack for convenient FBS storage
Your test tube racks aren’t just for conical tubes anymore. The One Shot FBS 50 mL bottle fits in most test tube racks—including strong, durable 30 mm Nalgene Unwire Racks. So, you get the convenience of fit in an easy-pour, easy-store, aliquot-free bottle.
Create less waste by eliminating FBS aliquots
The One Shot FBS 50 mL bottle generates 33% less waste than aliquoting from 500 mL bottles (Table 1). By using less plastic, less petroleum feedstock is consumed, and fewer greenhouse gas emissions are generated. By choosing One Shot FBS, a typical customer using 20 L of FBS per year would gain almost 7 hours back and generate 7 lb less plastic waste. This is a significant benefit both to lab productivity and the environment.
Table 1. Waste reduction using One Shot FBS 50 mL bottles versus aliquoting from 500 mL bottle
Method
Step in procedure
Plastic required
Weight (g)
Total plastic weight (g)
Aliquot from 500 mL bottle
Original container
1 x 500 mL bottle
79.4
Aliquot to conicals
1 x 25 mL serological pipette
18.8
10 conical tubes
140.3
238.5
One Shot FBS 50 mL Bottles
Original container
10 x 50 mL bottles
159.0
159.0
33% waste reduction
Pair for best performance
The 50 mL bottle size is a perfect match for use with a 500 mL bottle of Gibco RPMI, DMEM, or other Gibco cell culture media. This complete media pairing can help maximize performance and reproducibility.
For questions about the One Shot FBS 50 mL bottle's specifications, contact technical services at techsupport@thermofisher.com.
Available in single 50 mL bottle or 10-bottle pack
Choose a single 50 mL bottle or the One Shot carton, with a configuration of 10 x 50 mL.
Select the most cited FBS products in global scientific journals, packaged in convenient One Shot bottles.
A5670401,A5670402,A5669401,A5669402
Heat-inactivated (HI) FBS
Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.
Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.
Fetal Bovine Serum Basics Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.
Cell Culture Basics Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.
Cell Culture & Transfection Support Center Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
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For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.