NovaFluor dyes, including NovaFluor Yellow 610 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 610 dye can be used to replace PE/Dazzle™ 594, BD Horizon™ PE-CF594, or PE-Texas Red dye using the 561 nm yellow laser line for excitation. As compared with these PE tandem dyes, NovaFluor Yellow 610 dye shows much less cross-excitation and lower spectral spillover with the 488 nm blue laser line, allowing detection of an additional marker labeled with, for example, one of the NovaFluor Blue 610 dyes. The macromolecule-based NovaFluor Yellow 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.