Tools to study mitochondrial morphology and function—Multiplex your mitochondrial data
We highlight a few of our most referenced mitochondrial probes for studying cell morphology and function.
Find useful articles, videos and tools for studying cell structure using fluorescence microscopy and high-content analysis.
Cell structure features
On-demand webinar
Introduction to basic cytoskeleton labeling and detection
This webinar provides an overview of the cytoskeleton and includes a comprehensive guide to labeling and detection technologies such as live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
BioProbes article
Cell structure learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2011) | Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer | Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes |
| Application note (2015) | Collecting Z-stack image sequences with the EVOS FL Auto Imaging System | cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | Mitochondria in diseases—Note 12.2 | antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Lipid-mixing assays of membrane fusion—Note 13.1 | cell structure-plasma membrane, fluorescent lipids, membranes, micelles |
| Molecular Probes Handbook | Assays of volume change, membrane fusion and membrane permeability—Note 14.3 | cell structure-plasma membrane, fluorescent lipids, membranes, micelles |
| Molecular Probes Handbook | Antibodies for detecting membrane-surface labels—Note 13.2 | antibodies, cell structure-plasma membrane, extracellular proteins |
| Molecular Probes Handbook | Probes for the endoplasmic reticulum and golgi apparatus—Section 12.4 | antibodies, cell structure-er, cell structure-golgi, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for mitochondria—Section 12.2 | antibodies, cell structure-mitochondria, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 | antibodies, cell structure-lysosomes, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for the nucleus—Section 12.5 | antibodies, cell structure-nucleus, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability |
| Molecular Probes Handbook | A diverse selection of organelle probes—Section 12.1 | cell structure-er, cell structure-golgi, cell structure-lysosomes, cell structure-mitochondria, cell structure-nucleus, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Introduction to potentiometric probes—Section 22.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fast-response probes—Section 22.2 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential |
| Molecular Probes Handbook | Other nonpolar and amphiphilic probes—Section 13.5 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Introduction to membrane probes—Section 13.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fatty acid analogs and phospholipids—Section 13.2 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent fatty acid analogs, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Dialkylcarbocyanine and dialkylaminostyryl probes—Section 13.4 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Probes for neurotransmitter receptors—Section 16.2 | brain hormones, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, receptor binding |
| Molecular Probes Handbook | Probes for following receptor binding and phagocytosis—Section 16.1 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle |
| Molecular Probes Handbook | Probes for tubulin and other cytoskeletal proteins—Section 11.2 | cell structure-tubulin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Probes for actin—Section 11.1 | cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Nucleic acid stains—Section 8.1 | cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX |
| Molecular Probes Handbook | Tracers for membrane labeling—Section 14.4 | cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Protocol | TO-PRO-3 Stain | cell structure, imaging |
| Protocol | SYTOX Green Nucleic Acid Stain | cell structure, imaging |
| Protocol | SYTO 82 Nuclear Stain | cell structure, imaging |
| Protocol | SYTO 59 Nuclear Stain | cell structure, imaging |
| Protocol | NucRed Live 647 ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucRed Dead 647 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucGreen Dead 488 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucBlue Live ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucBlue Fixed Cell ReadyProbes Reagent | cell structure, imaging |
| Protocol | Hoechst 33342 for imaging | cell structure, imaging |
| Protocol | Hoechst 33342 for HCA instruments | cell structure, high content analysis |
| Protocol | HCS NuclearMask Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Deep Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Blue Stain | cell structure, high content analysis |
| Protocol | HCS CellMask Stains | cell structure, high content analysis |
| Protocol | DAPI for fluorescence imaging | cell structure, imaging |
| Protocol | DAPI for HCA instruments | cell structure, high content analysis |
| Protocol | ActinRed 555 ReadyProbes Reagent | cell structure, imaging |
| Protocol | ActinGreen 488 ReadyProbes Reagent | cell structure, imaging |
| Scientific poster (2009) | Cell-based assays for predictive hepatotoxicity measurements using high content imaging | Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability |
| Scientific poster (2009) | High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells | cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability |
| Scientific poster (2010) | Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis | Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2014) | New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy | autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader |
| Video | Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Staurosporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead). | cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | CellTracker Violet reagent and mitosis U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours. | cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Compilation of live cell imaging videos using Invitrogen fluorescent reagents This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes. | cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress |
| Video | Z Stack image of HeLa cells labeled with CellLights reagents A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm. | cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging |
| Video | Mitochondrial dynamics through cell division U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis. | cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Exosomes—The Next Small Thing: Episode 5—Collaboration—The key to scientific success | Exosomes |
| Video | Exosomes—The Next Small Thing: Episode 6—Exosomes the next small thing | Exosomes |
| Video | Exosomes—The Next Small Thing: Episode 4—Curiosity and a passion for science | Exosomes |
| Video | Exosomes—The Next Small Thing: Episode 3—Exosomes in cancer research | Exosomes |
| Video | Exosomes—The Next Small Thing: Episode 2—The history and promise of an exosome | Exosomes |
| Video | Exosomes—The Next Small Thing: Episode 1—What is an exosome? | Exosomes |
| Webinar | Introduction to basic cytoskeleton labeling and detection The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform. | cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
For Research Use Only. Not for use in diagnostic procedures.