Gibco Dynamis Medium provides the power to achieve higher titers, faster process development, and seamless scale-up.
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HEK293 cells were seeded at 0.3 x 106 cells/mL in Dynamis Medium supplemented with 4 mM L-Glutamine and cultured in three 60 mL shake flasks (125 rpm, 37°C, 8% CO2). In all flasks, viable cell density (VCD) was measured on days 0, 1, 2, 3, 4, and 7. By day 7, glucose was depleted in the culture. Glucose level monitoring and supplementation (e.g., 2 g/L glucose) at the beginning of the culture or as a supplement on day 4 is recommended. The results show consistent high-density growth performance with HEK293 cells in Dynamis Medium
A IgG-producing internal CHO clone was cultured in Dynamis Medium or other commercially available media for a period of up to 21 days with glucose levels maintained via supplementation. Titer values were normalized to 100% to the best competitor on Day 14. Not all media could sustain culture viability to the 21-day time point.
An IgG-producing CHO-DG44 external cell line was cultured in Dynamis Medium and EfficientFeed C+ Supplement and other commercially available media and feeds for a period of up to 18 days. Titer values normalized to 100% to the best competitor on Day 18.
AchtiCHO could not sustain culture viability to the 18-day time point. Balance CD CHO was close to Dynamis Medium in titer growth, but required 3 times more the feeding volume.
DG44 IgG producing cell line, when supplemented with just glucose over 21 days, showed that Dynamis Medium growth profile is unsurpassed for peak densities and duration of culture against competitors. Dynamis Medium reaches 10 million cells/mL faster and maintains that level of high viable cell densities longer than the comparative products shown.
HEK293 cells were seeded at 0.3 x 106 cells/mL in Dynamis Medium supplemented with 4 mM L-Glutamine and cultured in three 60 mL shake flasks (125 rpm, 37°C, 8% CO2). In all flasks, viable cell density (VCD) was measured on days 0, 1, 2, 3, 4, and 7. By day 7, glucose was depleted in the culture. Glucose level monitoring and supplementation (e.g., 2 g/L glucose) at the beginning of the culture or as a supplement on day 4 is recommended. The results show consistent high-density growth performance with HEK293 cells in Dynamis Medium
A IgG-producing internal CHO clone was cultured in Dynamis Medium or other commercially available media for a period of up to 21 days with glucose levels maintained via supplementation. Titer values were normalized to 100% to the best competitor on Day 14. Not all media could sustain culture viability to the 21-day time point.
An IgG-producing CHO-DG44 external cell line was cultured in Dynamis Medium and EfficientFeed C+ Supplement and other commercially available media and feeds for a period of up to 18 days. Titer values normalized to 100% to the best competitor on Day 18.
AchtiCHO could not sustain culture viability to the 18-day time point. Balance CD CHO was close to Dynamis Medium in titer growth, but required 3 times more the feeding volume.
DG44 IgG producing cell line, when supplemented with just glucose over 21 days, showed that Dynamis Medium growth profile is unsurpassed for peak densities and duration of culture against competitors. Dynamis Medium reaches 10 million cells/mL faster and maintains that level of high viable cell densities longer than the comparative products shown.
For faster scale-up, try Dynamis Medium in our AGT dry media format. AGT format offers simply reconstitution and animal origin free (AOF), chemically defined properties that help streamline regulatory processes.
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All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. BalanCD is a trademark of Irvine Scientific Sales Co. Cellvento is a trademark of Merck KGAA. EX-CELL is a trademark of SAFC Biosciences, Inc. HyCell and ActiCHO are trademarks of GE Healthcare Biosciences AB. PowerCHO is a trademark of Lonza. CHOZN is a trademark of Sigma-Aldrich Co.
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