Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.
To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker web tool.
Choose the right cellular pathway analysis assay for your research
| Analysis of an entire signal transduction pathway with a single end-point readout | Detection of post-translational modifications of specific protein substrates within a native cellular environment | Detection of post-translational modifications of specific protein substrates in the cell background of your choice | |||
|---|---|---|---|---|---|
| Stable vs. Transient | Stable | Stable | Transient | ||
| Cellular engineering | Pathway-specific response element upstream of BLA reporter | GFP-substrate fusion, readout of endogenous kinases | GFP-substrate fusion, readout of endogenous kinases | ||
| Assay optimization required? | No, already optimized | No, already optimized | Yes, if using cell backgrounds other than recommended | ||
| Detection technology | FRET (coumarin to fluorescein) | TR-FRET (terbium-labeled antibody to GFP-substrate fusion) | TR-FRET (terbium-labeled antibody to GFP-substrate fusion) | ||
| Antibody-based assay? |  | | |||
| Total assay volume (384-well) | 40 µL | 40 µL | 40 µL | ||
| Overcoming interference | Background suppression dye in the substrate mixture | Time-resolved readout allows read after interfering signals have decayed | Time-resolved readout allows read after interfering signals have decayed | ||
| Plate reader method | Bottom-read | Top-read | Top-read | ||
| Cost per well | $ | $$–$$$ | $$–$$$ | ||
| Use in SelectScreen® Cellular Profiling Services | | | | ||
| Request Info | Request Info | Request Info |
Elucidate your target pathways with multiple assay formats
Life Technologies offers three assay technologies for your cellular pathway analysis research.
- BacMam-enabled cellular assays are an efficient, easy-to-optimize, and robust method for interrogating specific signal transduction events in a cell background of choice.
- LanthaScreen cellular assays allow for detection of post-translational modifications of specific protein substrates within the native cellular environment.
- CellSensor pathway reporter gene assays measure the transcriptional activation of a pathway-specific cellular response element.
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