* 2012 Oracle™ Fusion Middleware Innovation Award.
** Instrument Management portal not available in all regions.
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QuantStudio 5 instrument |
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Sample capacity (wells) | 96 |
Reaction volume | 96-well: 0.2 mL block: 10–100 μL |
Footprint (W x D x H) Weight | 27 cm x 50 cm x 40 cm 25.7 kg (56.5 lbs) |
Excitation source | Complementary Metal-Oxide Semiconductor (CMOS) camera with Bright White LED |
Optical detection | 96 wells: 6 decoupled filters |
Excitation/detection range | 96 wells: 450–680 nm/500–730 nm |
Multiplexing | 96-well: up to 6 targets |
2D barcode reading | Optional |
Heating/cooling method | Peltier |
Temperature zone function | 96 wells: 6 Applied Biosystems™ VeriFlex™ zones |
Max block ramp rate | 6.5°C/sec |
Average sample ramp rate | 3.66°C/sec |
Temperature uniformity | 0.4°C |
Temperature accuracy | 0.25°C |
Run time | ~70 min for Quantifiler Trio and HP kits |
Dye compatibility (name) | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY*/NED/TAMRA/ Cy3, JUN*, ROX/Texas Red, MUSTANG PURPLE, Cy5/LIZ, Cy5.5 |
Chemistry capabilities | **Fast/standard |
Number of copies | 1 copy |
Sensitivity | Detect differences as small as 1.5-fold in target quantities in singleplex reactions |
* The instrument is factory calibrated with FAM, SYBR Green I, VIC, NED, ABY, JUN, MUSTANG PURPLE, TAMRA, Cy5, and ROX dyes. In order to run Quantifiler Trio or HP kits, HID ABY (Cat. No. 4461591), and HID JUN (Cat. No. 4461593) dyes will be delivered with each instrument and will require calibration upon installation.
** Not validated for human identification.
Please consult your Quantification User Guide for assay-specific parameters.
A34321,A34322,4482910,4482911,A31150,A31152,A31153,A31154,A78901,A78902