Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.
PureLink Purification Solutions
The Invitrogen PureLink nucleic acid purification kits are a family of purification products designed to meet the challenges posed by specific nucleic acid types, sample sources and volumes, throughput levels and downstream applications. These kits are based on either silica-membrane, anion exchange resin, or advanced silica-membrane purification technologies.
Learn more from our application notes:
PureLink Spin Column Kits
The PureLink spin column kits are a family of fast, simple, and cost-effective nucleic acid purification kits designed for the preparation of genomic DNA, plasmid DNA, total RNA, microRNAs and formalin-fixed paraffin-embedded (FFPE) RNA. These kits make use of proven silica membrane technology and eliminate the complex steps associated with older methodologies. Kits are available for both low-throughput (single samples) and high-throughput (96- and 384-sample) applications.
Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.
| Nucleic Acid | Application | Product |
|---|---|---|
| RNA | Viral RNA or DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants | PureLink Viral RNA/DNA Mini Kit |
| Total RNA from animal and plant cells, tissues, whole blood, yeast, bacteria | PureLink RNA Mini Kit | |
| Whole Blood | PureLink Total RNA Blood Kit | |
| Total RNA from bacteria, yeast, plants and mammalian cells—96 well | PureLink 96 RNA Purification System | |
| MicroRNA (miRNA) | PureLink miRNA Isolation Kit | |
| Total RNA from formalin-fixed paraffin-embedded (FFPE) samples | PureLink FFPE RNA Isolation Kit |
PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.
| Nucleic Acid | Application | Product |
|---|---|---|
| Plasmid DNA | Manual miniprep samples—Molecular Biology grade | PureLink Quick Plasmid Miniprep Kit |
| Manual miniprep samples—Transfection grade | PureLink HQ Mini Plasmid Purification Kits and PureLink HQ 96 Mini Plasmid Purification Kits | |
| High-throughput plasmid minipreps—96-well Transfection grade | PureLink HQ Mini Plasmid Purification Kits and PureLink HQ 96 Mini Plasmid Purification Kits | |
| Megaprep and Gigaprep—Transfection grade | PureLink HiPure Expi Plasmid Megaprep and Gigaprep Kits | |
| Sensitive cell lines; transfection of standard, robust cell lines; all molecular biology applications | PureLink Fast Low Endotoxin Plasmid Midi and Maxi |
Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.
PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.
| Nucleic Acid | Application | Product |
|---|---|---|
| Genomic DNA | Animal tissues and cells | PureLink Genomic DNA |
| Plant tissues | PureLink Genomic Plant DNA Purification Kit | |
| DNA | Agarose gel extraction | PureLink Gel Extraction Kit |
| PCR reaction clean-up | PureLink PCR Purification Kit |
Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.
Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.
| Nucleic Acid | Application | Product |
|---|---|---|
| RNA | Viral RNA or DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants | PureLink Viral RNA/DNA Mini Kit |
| Total RNA from animal and plant cells, tissues, whole blood, yeast, bacteria | PureLink RNA Mini Kit | |
| Whole Blood | PureLink Total RNA Blood Kit | |
| Total RNA from bacteria, yeast, plants and mammalian cells—96 well | PureLink 96 RNA Purification System | |
| MicroRNA (miRNA) | PureLink miRNA Isolation Kit | |
| Total RNA from formalin-fixed paraffin-embedded (FFPE) samples | PureLink FFPE RNA Isolation Kit |
Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.
PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.
| Nucleic Acid | Application | Product |
|---|---|---|
| Plasmid DNA | Manual miniprep samples—Molecular Biology grade | PureLink Quick Plasmid Miniprep Kit |
| Manual miniprep samples—Transfection grade | PureLink HQ Mini Plasmid Purification Kits and PureLink HQ 96 Mini Plasmid Purification Kits | |
| High-throughput plasmid minipreps—96-well Transfection grade | PureLink HQ Mini Plasmid Purification Kits and PureLink HQ 96 Mini Plasmid Purification Kits | |
| Megaprep and Gigaprep—Transfection grade | PureLink HiPure Expi Plasmid Megaprep and Gigaprep Kits | |
| Sensitive cell lines; transfection of standard, robust cell lines; all molecular biology applications | PureLink Fast Low Endotoxin Plasmid Midi and Maxi |
Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.
PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.
| Nucleic Acid | Application | Product |
|---|---|---|
| Genomic DNA | Animal tissues and cells | PureLink Genomic DNA |
| Plant tissues | PureLink Genomic Plant DNA Purification Kit | |
| DNA | Agarose gel extraction | PureLink Gel Extraction Kit |
| PCR reaction clean-up | PureLink PCR Purification Kit |
Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.
For additional information on our Plasmid Purification products go to Plasmid DNA Isolation
Select peer-reviewed publications
- Sathiamoorthy, S., Malott, R. J., Gisonni-Lex, L., et al. (2018). Selection and evaluation of an efficient method for the recovery of viral nucleic acid extraction from complex biologicals. NPJ vaccines, 3(1), 31.
- Bunker, J. J., Erickson, S. A., Flynn,et al. (2017). Natural polyreactive IgA antibodies coat the intestinal microbiota. Science, 358(6361), eaan6619.
- Solanas, G., Peixoto, F. O., Perdiguero, et al. (2017). Aged stem cells reprogram their daily rhythmic functions to adapt to stress. Cell, 170(4), 678-692.
- Sykes, D. B., Kfoury, Y. S., Mercier, F. Eet al. (2016). Inhibition of dihydroorotate dehydrogenase overcomes differentiation blockade in acute myeloid leukemia. Cell, 167(1), 171-186.
- Miyamoto T, Okano S, Kasai N. (2009) Irreversible thermoinactivation of ribonuclease-A by soft-hydrothermal processing. Biotechnol Prog. 25:1678-1685.
Shop for everyday lab essentials to power your PCR every step of the way
We've made it even easier for you to locate and purchase the products you need quickly to keep your work moving forward.
相關資源
技術支援
For Research Use Only. Not for use in diagnostic procedures.