Invitrogen™
PureLink™ 96 HQ Plasmid DNA Purification Kit
The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can Read more
Catalog number K210096
Price (USD)/ 4 x 96 preps
1,926.00
-
Price (USD)/ 4 x 96 preps
1,926.00
PureLink™ 96 HQ Plasmid DNA Purification Kit
Catalog numberK210096
Price (USD)/ 4 x 96 preps
1,926.00
-
The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.
The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection
Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.
The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection
Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format96-well Plate
Isolation TechnologySilica Plate
Sample TypeBacterial Culture
Final Product TypePlasmid DNA
For Use With (Equipment)Automated Liquid Handling Workstation
For Use With (Application)PCR, Transfection, Sequencing, Transformation, Cloning
High-throughput CompatibilityHigh-throughput Compatible
No. of Reactions4 x 96 Preps
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LinePureLink™
Product TypePlasmid DNA Purification Kit
Quantity4 x 96 preps
Shipping ConditionRoom Temperature
TargetPlasmid DNA
Test Time30 to 40 min.
Unit Size4 x 96 preps
Contents & Storage
Sufficient reagents are provided in the kit to perform 384 (4 x 96) isolations.
Reagents include:
• Resuspension Buffer (R1)
• Lysis Buffer (L1)
• Neutralization/Binding Buffer (B1)
• Wash Buffer (W1)
• Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1)
• RNase A
• PureLink HQ 96 Clarification Plate
• PureLink HQ 96 Binding Plate
• Receiver Plate
• Adhesive Foil for plates
Reagents include:
• Resuspension Buffer (R1)
• Lysis Buffer (L1)
• Neutralization/Binding Buffer (B1)
• Wash Buffer (W1)
• Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1)
• RNase A
• PureLink HQ 96 Clarification Plate
• PureLink HQ 96 Binding Plate
• Receiver Plate
• Adhesive Foil for plates