Download: Protein Gel Guide
The XCell SureLock Mini-Cell’s unique design allows you to run mini-gels quickly, easily, and leak-free without any clamps. The set-up time is only 30 seconds; just drop the Buffer Core and the Gel Tension Wedge into the Lower Buffer Chamber, insert the gels, and pull the Gel Tension Wedge forward. Tough polycarbonate construction of the unit boosts durability.
XCell SureLock Mini-Cell specifications
| Capacity | Up to 2 mini gels |
| Gel compatibility | Gel size: 8 x 8cm Gel cassette: 10 x 10cm Thickness: 1.0mm or 1.5mm |
| Compatible gels | All Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes |
| Buffer chamber Requirement | Upper chamber: 200 mL Lower chamber: 600 mL |
| Optional transfer apparatus | XCell II Blot Module |
| Transfer capacity | Up to 2 mini gels |
| Compatible power supplies | PowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001) |
| Unit dimensions | 14 x 13 x 16 cm (height with lid on) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 1,500 VDC or 75 Watts |
| Chemical resistance | Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
| Catalog number | EI0001 |
The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.
How XCell SureLock Mini-Cell works
The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.
 Locked Position |
 Unlocked Position |
Transfer using the XCell II Blot Module
The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.
Easily set up the XCell SureLock Mini-Cell in three simple steps
Step 1. Place one or two precast gels on buffer core.
Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.
Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.
Recommended electrophoresis run conditions
| Gel Type | Voltage (V) | Current (mA) | Ending Current | Run Time |
|---|---|---|---|---|
| Tris-glycine SDS-PAGE | 125 | 30-40 | 8-12 | 90 min |
| Tris-glycine Native | 125 | 6-12 | 3-6 | 1-12 hr |
| Bis-Tris (MES) | 200 | 110-125 | 70-80 | 35-50 min |
| Bis-Tris (MOPS) | 200 | 110-125 | 70-80 | 35-50 min |
| Tris-acetate SDS-PAGE | 150 | 40-55 | 25-40 | 50 min |
| Tricine | 125 | 80 | 40 | 90 min |
| Zymogram | 125 | 30-40 | 8-12 | 90 min |
| IEF pH 3-7 or pH 3-10 | 100-1 h 200-2 h 500- 30 min | 5 | 6 | 2.5 hr |
| TBE | 200 | 10-18 | 4-6 | 30-90 min |
| TBE-Urea | 180V | 10-20 | 6-14 | 50-75 min |
| DNA retardation | 100V | 12-15 | 6-15 | 90 min |
XCell SureLock Mini-Cell specifications
| Capacity | Up to 2 mini gels |
| Gel compatibility | Gel size: 8 x 8cm Gel cassette: 10 x 10cm Thickness: 1.0mm or 1.5mm |
| Compatible gels | All Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes |
| Buffer chamber Requirement | Upper chamber: 200 mL Lower chamber: 600 mL |
| Optional transfer apparatus | XCell II Blot Module |
| Transfer capacity | Up to 2 mini gels |
| Compatible power supplies | PowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001) |
| Unit dimensions | 14 x 13 x 16 cm (height with lid on) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 1,500 VDC or 75 Watts |
| Chemical resistance | Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
| Catalog number | EI0001 |
The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.
How XCell SureLock Mini-Cell works
The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.
Locked Position |
Unlocked Position |
Transfer using the XCell II Blot Module
The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.
Easily set up the XCell SureLock Mini-Cell in three simple steps
Step 1. Place one or two precast gels on buffer core.
Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.
Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.
Recommended electrophoresis run conditions
| Gel Type | Voltage (V) | Current (mA) | Ending Current | Run Time |
|---|---|---|---|---|
| Tris-glycine SDS-PAGE | 125 | 30-40 | 8-12 | 90 min |
| Tris-glycine Native | 125 | 6-12 | 3-6 | 1-12 hr |
| Bis-Tris (MES) | 200 | 110-125 | 70-80 | 35-50 min |
| Bis-Tris (MOPS) | 200 | 110-125 | 70-80 | 35-50 min |
| Tris-acetate SDS-PAGE | 150 | 40-55 | 25-40 | 50 min |
| Tricine | 125 | 80 | 40 | 90 min |
| Zymogram | 125 | 30-40 | 8-12 | 90 min |
| IEF pH 3-7 or pH 3-10 | 100-1 h 200-2 h 500- 30 min | 5 | 6 | 2.5 hr |
| TBE | 200 | 10-18 | 4-6 | 30-90 min |
| TBE-Urea | 180V | 10-20 | 6-14 | 50-75 min |
| DNA retardation | 100V | 12-15 | 6-15 | 90 min |
Resources
For Research Use Only. Not for use in diagnostic procedures.