We have several optimized cell-free expression systems for the rapid synthesis of recombinant proteins utilizing rabbit reticulocyte, or HeLa-based lysate systems. These mammalian systems can produce full-length and functionally active proteins, with the HeLa systems typically expressing higher protein yields than rabbit reticulocyte system.
- More choices—a variety of mammalian expression systems to accommodate different vectors, tags, yield requirements, functionality needs and cost considerations
- Fast—proteins can be expressed in as little as 90 minutes
- Easy-to-use—kits include all reagents and accessories needed for protein expression experiments
- High performance—kits are designed to maximize protein yield and/or function compared to similar products from other vendors
Featured mammalian cell-free protein expression products
Choose the right mammalian protein expression system for your experiment
| Retic Lysate IVT Kit | 1-Step Human Coupled IVT Kit | 1-Step Human High Yield IVT Kit | |
|---|---|---|---|
| Source | Rabbit reticulocyte | HeLa lysate | HeLa lysate |
| Yield | Low | Medium | High |
| Protein modifications | Limited glycosylation | Glycosylation and phosphorylation | Glycosylation and phosphorylation |
| Reaction time | 90 min | 90 min to 6 hr | 6–24 hr |
| Comments | Flexible system | First human-based system | Higher protein yield per reaction |
| Catalog # | 60 rxn: AM1200, 60 rxn w/manual AM1200M | DNA: 88881, 88882 | Mini: 88890, 88891 Maxi: 88892 |
Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized for use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes, into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection.
Ordering information for T7 cell-free expression vectors
For Research Use Only. Not for use in diagnostic procedures.