Plasmid DNA Isolation

適用於各種應用的質體DNA分離

我們的Invitrogen質體分離試劑盒提供您更多選擇，以降低多類型樣品處理過程所帶來的挑戰。我們的客製質體DNA萃取產品，可以依照您需要的純度和通量來分離質體DNA。我們提供一系列高效能且經濟實惠的質體純化技術，協助您克服常見的質體製備難題，如低回收率和過多雜質的問題。觀看我們的質體分離教學影片，了解如何使用Invitrogen PureLink和Thermo Scientific GeneJET萃取試劑盒來純化質體DNA。

When it comes to plasmid isolation, scientists have a range of options depending on their specific needs. Molecular-grade plasmid isolation technologies offer speed and cost-effectiveness, making them excellent for robust applications like cloning, nucleic acid labeling, PCR, and sequencing, where high purity levels are not essential. On the other hand, transfection-grade plasmid purification technologies are designed for applications that require greater purity and higher yields, such as transfection of standard cell lines and in vitro transcription. Transfection-grade plasmid DNA can also be used for molecular-grade applications. For the most sensitive applications, such as transfection of sensitive cell lines or in vivo research, advanced transfection-grade plasmid isolation technology is recommended. This technology helps ensure the highest purity and can also be used for all molecular and transfection-grade applications.

Learn more: Plasmid DNA purity grades

為了達成下游應用需要的純度以及產量，可獲得穩定的質體DNA純化結束是蛋白質表現工作流程的關鍵步驟。了解如何獲取轉染等級的質體，接續可應用於Gibco ExpiCHO和Expi293的哺乳動物表現系統。

下載DNA指南   下載應用指引   下載產品選擇指南

Explore: ExpiCHO Expression System

Automated plasmid purification is an advanced technology that utilizes robotics to streamline the process. Thermo Fisher provides automated plasmid purification solutions that offer high-throughput and centrifuge-free purification of transfection-grade plasmid DNA. These solutions minimize human error and help ensure reproducibility by precisely controlling purification steps and reagent volumes. For mini scale applications, specialized magnetic-bead purification kits are available for efficient plasmid DNA isolation on KingFisher platforms with liquid handling capabilities. Likewise, for maxi scale automation, the KingFisher PlasmidPro maxi processer offers a hands-free experience with prefilled cartridges. The purified plasmid DNA obtained through this process can be used for a range of downstream applications, including transfection, vector preparation, enzymatic modifications, cloning, sequencing, and in vitro transcription/translation.

Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively impact cell growth, cell viability, transfection efficiency, protein expression, and even mimic sepsis. To address these concerns, our kits designed for low-endotoxin plasmid DNA isolation carefully remove these endotoxins, helping ensure the integrity of the isolated plasmid DNA. These kits offer a fast and easy method of plasmid purification, helping provide high purity and yield for sensitive downstream applications. Whether you are working with small or large prep sizes, our transfection-grade plasmid isolation kits are tailored to help meet your specific needs, enabling you achieve reliable and reproducible results. 

For scientists who require an even higher level of endotoxin removal, we offer advanced transfection-grade plasmid isolation kits. These kits go beyond the standard transfection-grade kits and provide an enhanced level of endotoxin removal, helping ensure the utmost purity of the isolated plasmid DNA. With our advanced transfection-grade plasmid isolation kits, you can confidently perform downstream applications that demand the highest quality pDNA. These kits are excellent for researchers working on critical experiments where even trace amounts of endotoxins can have a significant impact on their results. Using our advanced-transfection grade plasmid isolation kits can help eliminate the risks associated with endotoxins and obtain pure plasmid DNA for your scientific endeavors.

For fast, easy, and reliable plasmid purification, our Invitrogen PureLink and GeneJET molecular-grade plasmid DNA (pDNA) isolation kits produce pure, ready-to-use plasmids that are compatible with a range of standard molecular biology applications. Molecular-grade kits are suitable for transformation of bacterial and yeast cells; however, they may not be well suited for transfection of sensitive mammalian cells due to the presence of endotoxins. For transfection applications, please see the transfection-grade plasmid purification kits above.

• PureLink HiPure BAC Buffer Kit
• PureLink Expi Endotoxin-Free Buffer Set