It is critical to know your options for flow cytometry instrumentation and to understand how your instrument works. This section provides educational resources about these instruments including guided learning on the basics of your flow cytometer, a guide on how to evaluate an instrument when considering a new purchase, as well as videos, application notes, webinars and more.
Flow cytometry instrumentation features
Guided learning
Fundamentals of Flow Cytometry-How a flow cytometer works in Molecular Probes School of Fluorescence
This guided learning module covers an introduction to flow cytometer and goes over the basics of the flow cytometer and how it works.
Evaluation guide
Flow cytometer evaluation guide
The evaluation guide for flow cytometers will help you compare various flow cytometers and learn about what to consider specifically to fulfill your applications needs, sample types and volumes, cells of interest, and research goals.
Learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2019) | Development of in vitro immune effector function assays to better approximate the in vivo behavior of biotherapeutics and cell therapies | Attune NxT, flow cytometer, CellInsight CX7, CellTrace dyes, cell therapies, DynaBeads, cytotoxicity |
| Application note (2019) | Functional Flow Cytometry to predict PD-L1 Conformational Change and Improve Cancer Immunotherapy | Attune NxT, flow cytometer, programmed death ligand, PD-L1, cancer immunotherapy |
| Application note (2018) | Using the Attune NxT Flow Cytometer for rapid and accurate analysis of nuclear DNA content in plants | Attune NxT Flow Cytometer, plants |
| Application note (2018) | High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates | Attune NxT Flow Cytometer, Autosampler |
| Application note (2018) | A guide to analyze Attune NxT FCS data files in FCS Express 6 Software | Attune NxT Flow Cytometer, Attune NxT software, flow cytometry |
| Application note (2017) | Recommendations for accurate concentration measurements on the Attune NxT Flow Cytometer | Attune NxT Flow Cytometer, flow cytometry, cell concentration, cell counting |
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research |
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| Application note (2017) | Flow cytometry analysis of dose response for apoptosis induction | apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green |
| Application note (2017) | Detection of platelets in whole blood using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse |
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Application note (2015) | Live/dead algal analysis with the Attune NxT Flow Cytometer | algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability |
| Application note (2015) | No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer | antibodies, Attune NxT, flow cytometer, flow cytometry sample preparation, fluorescent dyes, immunology, immunophenotyping, particles, pH detection |
| BioProbes article (2017) | Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometer | apoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability |
| BioProbes article (2016) | High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometry | Attune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability |
| BioProbes article (2016) | Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research |
| BioProbes article (2015) | Journal Club—Multiparameter detection of early apoptosis markers without compensation | apoptosis, Attune NxT, cell structure-mitochondria, flow cytometry compensation, fluorescence, immunophenotyping |
| BioProbes article (2015) | Multiparameter detection of fluorescent protein expression with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, flow cytometry assays, fluorescence, fluorescent proteins, multicolor flow cytometry |
| BioProbes article (2015) | No-compensation immunophenotyping using a four-laser flow cytometer | Attune NxT, flow cytometer, fluorescence, immunophenotyping, multicolor flow cytometry |
| BioProbes article (2015) | No-wash, no-lyse assays for the Attune NxT Flow Cytometer | Attune NxT, endocytosis, flow cytometer, flow cytometry assays, fluorescence, immunophenotyping, multicolor flow cytometry, phagocytosis |
| BioProbes article (2015) | The next generation in flow cytometry—The Attune NxT Flow Cytometer | antibodies, Attune NxT, flow cytometer, fluorescence |
| BioProbes article (2015) | Tools and strategies for rare-event detection using flow cytometry | Attune NxT, flow cytometer, fluorescence, rare cell detection, rare event detection |
| BioProbes article (2014) | The next generation in acoustic focusing cytometry is here—The Attune NxT Acoustic Focusing Cytometer | Attune NxT, flow cytometer |
| BioProbes article (2010) | Advanced cell proliferation analysis | Attune NxT cell proliferation, cell tracking, flow cytometry, fluorescence |
| Blog post | Clog resistance of non-pressure based flow cytometers | Attune NxT, flow cytometer |
| Blog post | What are event rates and should you always trust the “Maximum Event Rate” of your flow cytometer? | Attune NxT, flow cytometer, instrument set-up |
| Blog post | Flow cytometer fluidics and why they are not all the same | Attune NxT, flow cytometer, instrument set-up |
| Guided learning | Molecular Probes School of Fluorescence—Flow Cytometry Basics | flow cytometry, flow cytometer, fluidics, optics, electronics, flow cytometry basics |
| Guided learning | Fundamentals of Flow Cytometry-How a flow cytometer works in Molecular Probes School of Fluorescence | flow cytometer |
| Guided learning | Fundamentals of Flow Cytometry-Fluidics of a flow cytometer in Molecular Probes School of Fluorescence | flow cytometer |
| Guided learning | Fundamentals of Flow Cytometry-Optics of a flow cytometer in Molecular Probes School of Fluorescence | flow cytometer |
| Guided learning | Fundamentals of Flow Cytometry-Electronics of a flow cytometer in Molecular Probes School of Fluorescence | flow cytometer |
| Molecular Probes Handbook | Flow cytometry reference standards—Section 23.2 | flow cytometer |
| Scientific Poster (2022) | A rapid method for assessing the accumulation of nanoplastics in human peripheral blood | Flow cytometry |
| Scientific Poster (2022) | PD-L1 expression in NSCLC MDSCs and its potential use as a biomarker to determine treatment response through dimensionality reduction of flow cytometric data | Flow cytometry |
| Scientific poster (2018) | Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage | Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research |
| Scientific poster (2018) | Evaluation of multiple techniques for PMT optimization | Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings |
| Scientific poster (2018) | Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia | Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry |
| Scientific poster (2018) | Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques | Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination |
| Scientific poster (2018) | Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes | academic research solutions, microplates, quality control, temperature control |
| Scientific Poster (2017) | T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes | antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright |
| Scientific Poster (2016) | An objective test to qualify plate samplers for complex flow cytometric assays | Attune NxT, Autosampler, flow cytometer |
| Scientific Poster (2016) | Cell mediated cytotoxicity in “untouched” whole blood | Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood |
| Scientific Poster (2016) | No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads | antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes |
| Scientific Poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2014) | Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool | Attune NxT, drug discovery, flow cytometer, flow cytometry assay |
| Scientific poster (2011) | Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells | Alexa Fluor, endothelial cells, flow cytometry, fluorescent dyes, rare event detection |
| CYToMORE lecture video (2016) | ...and on the 8th day, God created cytometry Presented by J. Paul Robinson, Purdue University, USA | flow cytometers, flow cytometry, history, technology developments |
| CYToMORE lecture video (2016) | High throughput and high content flow cytometry Presented by J. Paul Robinson, Purdue University, USA | Attune NxT, flow cytometer, high content flow cytometry, high throughput screening |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | mass cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Rare cells analysis: from bench to clinics Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy | Attune NxT, flow cytometer, rare cell detection, rare event detection |
| CYToMORE lecture video (2016) | Software tutorial: what’s new Presented by Carmen Gondhalekar, Purdue University, USA | Attune NxT, flow cytometer, software |
| CYToMORE lecture video (2016) | Tuning acoustic forces for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometer |
| Webinar | How recent technological advances in flow cytometry instrumentation are enabling faster throughput This webinar presented by J. Paul Robinson at Purdue University, will focus on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT instrument fits in current and future applications. | Attune NxT, cell cycle, multicolor flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data | flow cytometer calibration, flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra | absorbance, emission, excitation, spectra |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Flow Cytometry | flow cytometer calibration, multicolor flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Fluorescence | flow cytometry, fluorescence imaging |
| Tutorial video | Molecular Probes Tutorial Series—Overview of Filters and Light Sources | emission, excitation, filters, light cubes, light sources |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.