In this collection, protocols for each antibody application are provided along with links to antibodies, reagents, and resources. Learn how to perform each application so quality, consistent results are attained every time.
Western blot (WB) is a technique that separates a mixture of proteins via size exclusion electrophoresis. The target protein is identified using an antibody specific for the target and is visualized as a band via chemiluminescent or fluorescent detection.
Shop WB antibodies | Shop WB reagents | Blot Builder | WB troubleshooting
Western blot antibody dilution calculator
Flow cytometry identifies cellular characteristics and expression of proteins in a heterogeneous cell population by flowing one cell at a time past a laser beam.
Shop Flow antibodies | Shop Flow reagents | Panel Builder | SpectraViewer
Immunohistochemistry (IHC) identifies structures in whole tissue samples. A mixture of antibodies and stains are used to microscopically visualize the cells.
Shop IHC antibodies | IHC troubleshooting
Download IHC guide
Immunocytochemistry/Immunofluorescence (ICC/IF) identifies cells from samples that are no longer in their natural state. Antibodies are used to microscopically visualize cellular structures.
Shop ICC/IF antibodies | ICC/IF troubleshooting | SpectraViewer
Enzyme-linked immunosorbent assay (ELISA) identifies protein concentrations using antibodies and a plate with multiple sample wells. Visualization is performed using colorimetric or fluorescent enzymatic substrates and is represented graphically.
ELISA Overview | Shop ELISA antibodies | Shop ELISA kits | Shop ELISA reagents
Download Biomarker guide
Chromatin immunoprecipitation (ChIP) analyzes protein-DNA interactions. The target interactions are identified using antibodies and the DNA is amplified for graphical visualization.
Shop ChIP antibodies
Download ChIP guide | Protein preparation handbook
Immunoprecipitation (IP) precipitates a target protein from a mixture of proteins using an antibody. Immunoprecipitation can be used in conjunction with other assays or independently.
Shop IP antibodies | Shop IP beads, resins, kits | IP troubleshooting
Protein preparation handbook
For Research Use Only. Not for use in diagnostic procedures.
