Mouse cerebellum section. NeuroTrace® 530/615 red-fluorescent Nissl stain and nuclear yellow.
Neurons in a mouse cerebellum section labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain (Cat. no. N21482). The Nissl substance, ribosomal RNA associated with the rough endoplasmic reticulum, is specific to neuronal cells. Other cells in the sample are identified with the contrasting nuclear counterstain nuclear yellow (Cat. no. N21485). Smaller neurons appear to be labeled primarily with the nuclear yellow stain. This image is a composite of two micrographs acquired using a DAPI longpass filter set and a filter set appropriate for tetramethylrhodamine.
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