Spontaneous intracellular Ca2+ fluctuations of neurons developing in vivo.
Spontaneous intracellular Ca2+ fluctuations of neurons developing in vivo. The spinal cord was dissected from a neurula-stage Xenopus embryo and loaded with fluo-3 AM (Cat. no. F1241, F1242, F14218, F14242, F23915). Regions of fluo-3 fluorescence on the ventral side of the spinal cord are presented pseudocolored in gold and indicate areas of highest intracellular Ca2+ (Nature 375, 784 (1995)). The image was obtained with a Bio-Rad MRC600 confocal laser-scanning microscope. The image was contributed by Nicholas C. Spitzer, University of California, San Diego.
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