Easier ELISA Workflows
Learn how Invitrogen Instant ELISA kits can streamline your ELISA workflow.
Purchase any of our Invitrogen Instant ELISA kits at 35% off!
Valid till 30 September 2021.
Valid until 30 Sept 2021
Ending in:dayhourminsec
Targets available
Included Kit components
- Precoated 96-well plate
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard (in addition well strips)
- Stop solution
- Substrate solution
- Assay buffer (sample diluent)
- Wash buffer
- Lysis buffer
In conventional ELISA kits, the precoated plates provide only the capture antibody, whereas Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including lyophilized protein standards, capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol.
| 17 steps for conventional ELISA | 7 steps for Instant ELISA kits | ||
| 1 | Washing of coated plate | ||
| 2 | Reconstitution of standard protein | ||
| 3 | Addition of sample diluent to standard wells | ||
| 4 | Titration of standard curve | 1 | Rehydration of plate |
| 5 | Addition of sample diluent | 2 | |
| 6 | Sample addition | 4 | |
| 7 | Dilution of biotin conjugate | ||
| 8 | Addition of biotin conjugate | ||
| 9 | Incubation | ||
| 10 | Preparation of streptavidin-HRP conjugate | ||
| 11 | Washing step | ||
| 12 | HRP conjugate | ||
| 13 | Incubation | ||
| 14 | Washing step | 4 | Washing step |
| 15 | Addition of TMB substrate | 5 | Addition of TMB substrate |
| 16 | Addition of stop solution | 6 | Addition of stop solution |
| 17 | Calculation of results | 7 | Calculation of results |
By using an Instant ELISA kit, there are only 7 working steps, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
Articles and technical guides
Time-saving immunoassay tools
Find the right immunoassay for your research needs.
Biomarker quantitation assay guide
Immunoassay guide to protein quantitation.
ELISA related products
ELISA reagents and buffers
Wash buffers, blocking buffers and enzyme substrates.
ELISA special services
Request bulk packaging, lot reservation or custom kits.
Additional products
ProcartaPlex multiplex assays
Multiplex immunoassays using the Luminex platform.
ELISA special services
Request bulk packaging, lot reservation or custom kits.
Antibodies
We offer a variety of specific antibodies to build your own ELISA.
For Research Use Only. Not for use in diagnostic procedures.
Terms and Conditions
This promotion is open only to customers in Singapore. Customers who purchase any Invitrogen Instant ELISA kits will receive 35% off list price. In order to enjoy the 35% discount, the promotion code RELI20 must be presented at time of order no later than September 30, 2021, or before promotional supplies are depleted, whichever comes first. Healthcare professionals may not participate in this promotion. Offer void where prohibited, licensed, or restricted by state laws or regulation, or agency/ institutional policy. Other restrictions may apply.
The Company reserves the right to change these terms and conditions at any time without prior notice.