High-quality genomic coverage from nasopharyngeal, oropharyngeal, and diverse samples increases accuracy in SARS-CoV-2 research such as the study of viral evolution and transmission patterns. The optimized workflow for SARS-CoV-2 reduces time and sequencing costs while providing flexibility to use available reagents.
Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.
- PCR-free protocols
- Best coverage of challenging genomic regions with the physical shearing protocol
- Improved sensitivity of variant detection from 1 ng of DNA compared to Illumina, Kapa Biosystems™, or NEB™ kits
- Visual feedback for higher library prep success rates
- Protocols available for DNA that is physically sheared (PS) or enzymatically sheared (ES)
Accommodates a wide range of sample types and inputs:
- Intact DNA
- Degraded DNA, including FFPE or cfDNA
- Suitable for genomes of all sizes
- Consistent gene coverage from 1 to 500–1,000 ng
Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.
Sequence SARS-CoV-2 samples
Physically sheared DNA
Sequencing studies can now accept a greater diversity of samples and retain confidence in genomic interpretation. Variant detection rates by the Invitrogen Collibri PS DNA Library Prep Kits for Illumina Systems are similar between amplified
Figure 1. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
Enzymatically sheared DNA
Consistency of variant detection provides more consistent, predictable results from diverse samples. Variant detection rates by the Invitrogen Collibri ES DNA Library Prep Kits for Illumina Systems are similar between amplified (100 ng) libraries and PCR-free (100 ng) libraries.
Figure 2. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina NovaSeq 6000 system at 258M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
Improve statistical power of NGS results through miniaturization
“…in a 1/10th reaction with Collibri preps, you can see really tight performance.”
Dr. Stuart Levine, Director of the BioMicro Center, MIT
Product comparison
 Collibri PS DNA Library Prep Kits for Illumina Systems |  Collibri ES DNA Library Prep Kits for Illumina Systems | |
| PCR-free input | 500 ng | 100 ng |
| Input | 1–1,000 ng | 1–500 ng |
| Lowest GC bias | ||
| DNA shearing method | Physical shearing (e.g., Covaris) | Enzymatic shearing |
| Species supported | All | All |
| PCR-free assay time (hr) | 1.5 | 1.5 |
| Assay time (hr) | 2.75 | 2.5 |
| PCR-free hands-on time (hr) | 0.5 | 0.5 |
| Hands-on time (hr) | 1 | 1 |
| Target insert size (bp) | 350-550 | 350-550 |
| All components included | ||
| Visual feedback | ||
| View performance data | View performance data |
Technical Note: DNA fragmentation strategies for next-generation sequencing library preparation
Choose between DNA fragmentation options based upon the need for even genomic coverage, high yields, and ease-of-automation.
Ordering information
Resources
App note
Brochures
DNA sequencing
Technical notes
All sequencing methods
Technical notes
RNA sequencing
Technical notes
For Research Use Only. Not for use in diagnostic procedures.