De Novo Sequencing by Sanger Sequencing

de novo sequencing is the term used to describe the initial sequence analysis performed to obtain the primary genetic sequence of a particular organism. A detailed genetic analysis of an organism is possible only after de novo sequencing has been performed.

For de novo sequencing using capillary electrophoresis, the target DNA is fragmented and cloned into a viral or plasmid vector. Cloning provides amplification of the target DNA (by bacterial growth) and allows sequencing primers to bind to known sequence in the vector and extend the sequence into the unknown target DNA. de novo sequencing methods are described in the Sequencing Chemistry Guide page 16-20.