Download: Protein Gel Guide
Recommended loading volumes per well for mini gels
Standard gel combs
| Recommended loading volume* | Maximum protein load per band | DNA per band | ||
|---|---|---|---|---|
| Well format | 1.0 mm thickness | 1.5 mm thickness | ||
| 1-well | 700 μL | - | 12 µg | 2.4 µg |
| 5-well | 60 μL | - | 2 µg | 400 ng |
| 9-well | 28 μL | - | 0.5 µg | 100 ng |
| 10-well | 25 µL | 37 µL | 0.5 µg | 100 ng |
| 12-well | 20 µL | - | 0.5 µg | 100 ng |
| 15-well | 15 µL | 25 µL | 0.5 µg | 100 ng |
| 17-well | 15 μL | - | 0.5 µg | - |
| IPG | 7 cm IPG strip | - | - | - |
| 2D-well | 400 µL | 600 μL | 12 µg | 2.0 µg |
* Recommended loading volume represents ~60% of maximum loading volume
WedgeWell combs
(e.g. Bolt Bis-Tris Plus Mini Gels and Novex Tris-Glycine mini precast gels)
| Well format | Recommended loading volume | Maximum loading volume | Maximum protein load per band |
|---|---|---|---|
| 10-well WedgeWell | 40 μL | 60 μL | 0.5 µg |
| 12-well Wedgewell | 30 μL | 45 μL | 0.4 µg |
| 15-well Wedgewell | 20 μL | 35 μL | 0.25 µg |
| 17-well Wedgewell | 15 μL | 30 μL | 0.2 µg |
Recommended loading volumes per well for midi gels
| Well format | Recommended loading volume* | Maximum loading volume | Maximum protein load per band for silver staining |
|---|---|---|---|
| 12+2-well | 45 μL + 15 μL | 60.7 μg | 1.4 ng |
| 20-well | 25 μL | 0.5 μg | 1 ng |
| 26-well | 15 μL | 0.35 μg | 0.7 ng |
* Recommended loading volume represents ~60% of maximum loading volume
Resources
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