Class-Specific Protein Quantitation | Thermo Fisher Scientific

packaging and reagents for protein quantitation

These specialized protein assay reagents and kits allow you to estimate and measure specific post-translational modification (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins and phosphoproteins.

Technical handbook

Estimation kits
Ordering

Glycoprotein Carbohydrate Estimation Kit		Phosphoprotein Phosphate Estimation Assay Kit

Pierce Glycoprotein Carbohydrate Estimation Kit	Glycoprotein Standards Set for Pierce Glycoprotein Carbohydrate Estimation Kit	Pierce Phosphoprotein Phosphate Estimation Assay Kit
Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).		Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.
Assay overview Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars Semiquantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards Simple procedure—completed in less than 75 minutes; All you need is this kit, a microplate, and a plate reader to determine carbohydrate content Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components		Assay overview Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr) Convenient—test tube and 96-well microplate protocols included and requires less than 90 minutes to perform Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available
Applications Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents Characterize and classify purified protein fractions from an affinity procedure Assess sugar and carbohydrate contamination in non-glycosylated protein samples		Applications Identify proteins as containing phosphoserine or phosphothreonine phosphorylations Estimate the amount of pS and pT phosphorylation Develop specific quantitative assays for well-characterized phosphoproteins of interest
23260	23259	23270

Estimation kits

Glycoprotein Carbohydrate Estimation Kit		Phosphoprotein Phosphate Estimation Assay Kit

Pierce Glycoprotein Carbohydrate Estimation Kit	Glycoprotein Standards Set for Pierce Glycoprotein Carbohydrate Estimation Kit	Pierce Phosphoprotein Phosphate Estimation Assay Kit
Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).		Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.
Assay overview Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars Semiquantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards Simple procedure—completed in less than 75 minutes; All you need is this kit, a microplate, and a plate reader to determine carbohydrate content Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components		Assay overview Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr) Convenient—test tube and 96-well microplate protocols included and requires less than 90 minutes to perform Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available
Applications Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents Characterize and classify purified protein fractions from an affinity procedure Assess sugar and carbohydrate contamination in non-glycosylated protein samples		Applications Identify proteins as containing phosphoserine or phosphothreonine phosphorylations Estimate the amount of pS and pT phosphorylation Develop specific quantitative assays for well-characterized phosphoproteins of interest
23260	23259	23270

Ordering

Resources

Pierce Protein methods – an encyclopedia of articles about protein analysis

Protein assay technical guide – tools and reagents for improved quantitation of total or specific proteins.

Protein assay compatibility table – summarizes compatible substances for several popular protein assays.

Protein Analysis Learning Center – resources for different protein analysis techniques

Protein Biology Application Notes – journal-style articles based on research applications and proof-of-concept experiments

For Research Use Only. Not for use in diagnostic procedures.