Quick links
- Forward, Reverse, Repetitive, & Heterogeneous Sample Pipetting
- Pipetting Recommendations for Different Sample Types
- 10 Steps to Improve Pipetting Accuracy
- Pipette Accuracy vs. Precision
- Factors Affecting Pipette Accuracy
Complete the form and download a pdf version of the GLP Guide.
Pipette-to-sample
A contaminated pipette or contaminated tips can cause contamination of samples.
Prevention:
- Use filter tips.
- Change the tip after pipetting each sample.
- Clean the pipette regularly.
Sample-to-pipette
Samples or aerosols from samples can enter the cone of the pipette.
Prevention:
- Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body.
- Release the push button slowly.
- To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.
Sample-to-sample (carry-over)
The remains of a sample can mix with the next sample inside the tip and may cause a false test result.
Prevention:
- Change the tip after each sample.
- If you suspect your pipette is contaminated, clean with a suitable method, and autoclave if needed.
