Biotin-labeled secondary antibodies are commonly used in various applications such as western blotting, ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and flow cytometry. Secondary antibodies labeled with multiple biotin molecules, when combined with a streptavidin- or avidin-based conjugates, allow for signal amplification of lowly expressed proteins. Search below our wide-ranging catalog of biotinylated secondary antibodies that can be used with fluorescent or enzymatic streptavidin- or avidin-based techniques.
Biotinylated secondary antibodies by application
Navigate the tables below to see our wide-ranging offerings for biotinylated secondary antibodies by application.
Need a fluorescent substrate for immunofluorescence, immunohistochemistry, or in situ hybridization applications? Check out our Tyramide SuperBoost Kits to amplify your signal!
Table 1. Biotinylated secondary antibodies for western blotting
Table 2. Biotinylated secondary antibodies for ELISA applications
Table 3. Biotinylated secondary antibodies for IHC
Table 4. Biotinylated secondary antibodies for ICC
Target species | Host | ||||||
---|---|---|---|---|---|---|---|
Goat | Rabbit | Mouse | Donkey | Rat | Bovine | Horse | |
Anti-Mouse | Biotin labeled goat anti-mouse | Biotin labeled rabbit anti-mouse | — | — | Biotin labeled rat anti-mouse | — | Biotin labeled horse anti-mouse |
Anti-Human | Biotin labeled goat anti-human | Biotin labeled rabbit anti-human | Biotin labeled mouse anti-human | — | — | Biotin labeled bovine anti-human | — |
Anti-Rabbit | Biotin labeled goat anti-rabbit | — | Biotin labeled mouse anti-rabbit | Biotin labeled donkey anti-rabbit | — | — | — |
Anti-Rat | Biotin labeled goat anti-rat | Biotin labeled rabbit anti-rat | Biotin labeled mouse anti-rat | — | — | — | — |
Anti-Goat | — | Biotin labeled rabbit anti-goat | Biotin labeled mouse anti-goat | — | — | — | — |
Anti-Chicken | — | Biotin labeled rabbit anti-chicken | — | — | — | Biotin labeled bovine anti-chicken | — |
Anti-Hamster | Biotin labeled goat anti-hamster | — | — | — | — | — | — |
Anti-Sheep | — | Biotin labeled rabbit anti-sheep | — | — | — | — | — |
Anti-Horse | Biotin labeled goat anti-horse | — | — | — | — | — | — |
Table 5. Biotinylated secondary antibodies for immunofluorescence applications
Table 6. Biotinylated secondary antibodies for flow cytometry
Table 1. Biotinylated secondary antibodies for western blotting
Table 2. Biotinylated secondary antibodies for ELISA applications
Table 3. Biotinylated secondary antibodies for IHC
Table 4. Biotinylated secondary antibodies for ICC
Target species | Host | ||||||
---|---|---|---|---|---|---|---|
Goat | Rabbit | Mouse | Donkey | Rat | Bovine | Horse | |
Anti-Mouse | Biotin labeled goat anti-mouse | Biotin labeled rabbit anti-mouse | — | — | Biotin labeled rat anti-mouse | — | Biotin labeled horse anti-mouse |
Anti-Human | Biotin labeled goat anti-human | Biotin labeled rabbit anti-human | Biotin labeled mouse anti-human | — | — | Biotin labeled bovine anti-human | — |
Anti-Rabbit | Biotin labeled goat anti-rabbit | — | Biotin labeled mouse anti-rabbit | Biotin labeled donkey anti-rabbit | — | — | — |
Anti-Rat | Biotin labeled goat anti-rat | Biotin labeled rabbit anti-rat | Biotin labeled mouse anti-rat | — | — | — | — |
Anti-Goat | — | Biotin labeled rabbit anti-goat | Biotin labeled mouse anti-goat | — | — | — | — |
Anti-Chicken | — | Biotin labeled rabbit anti-chicken | — | — | — | Biotin labeled bovine anti-chicken | — |
Anti-Hamster | Biotin labeled goat anti-hamster | — | — | — | — | — | — |
Anti-Sheep | — | Biotin labeled rabbit anti-sheep | — | — | — | — | — |
Anti-Horse | Biotin labeled goat anti-horse | — | — | — | — | — | — |
Table 5. Biotinylated secondary antibodies for immunofluorescence applications
Table 6. Biotinylated secondary antibodies for flow cytometry
Biotinylated reagents for improved protein binding
See all streptavidin/biotin binding protein conjugates for imaging or flow cytometry
Find Streptavidin Dynabeads for purification of protein or isolation of cells
Learn more about the Endogenous Biotin-Blocking Kit
Tyramide SuperBoost technology for use with biotin labeled secondary antibodies
Find all Alexa Fluor streptavidin and avidin conjugates for biotin binding
Amplify the signal of your biotin labeled antibody using the Biotin XX Tyramide SuperBoost Kit, Streptavidin (Cat. No. B40931). This specific kit contains biotin XX tyramide with HRP-conjugated streptavidin, which is used prior to detection and signal amplification with an Alexa Fluor-conjugated streptavidin molecule. The table below provides various Alexa Fluor-streptavidin conjugates for use with Biotin XX Tyramide SuperBoost Kit, Streptavidin.
Figure 1. ATP Synthase detection with Biotin XX Tyramide SuperBoost Kit. HeLa cells were fixed and permeabilized with Image-iT Fixation/Permeabilization Kit (Cat. No R37602). For ATP synthase detection, cells were labeled with anti-ATP Synthase primary antibody and a biotin-conjugated secondary antibody. Biotin signal was enhanced using Biotin XX Tyramide SuperBoost Kit – Streptavidin (Cat. No B40931) and detected with Alexa Fluor 488 Streptavidin (Cat. No. S11223). For best antifade protection, cover slip was mounted with ProLong Diamond Antifade Mountant (Cat. No. P36961).
For Research Use Only. Not for use in diagnostic procedures.