Berthold Technologies Mithras² LB 943 and Tristar² S LB 942 have been rigorously tested using either the Terbium Assay Application Note or the Europium Assay Application Note with demonstrated performance as shown in the setup guide.
Before using LanthaScreen Terbium and Europium Assays
Test your plate reader setup
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.
Berthold Technologies Mithras² LB 943 instrument configuration
| Assay technology | Adapta and LanthaScreen Eu | GeneBLAzer | LanthaScreen Tb | Predictor | Z'-LYTE |
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | Mithras2 LB 943 Microplate Reader Europium (Eu) Assay Setup Guide | Compatible, not tested | Mithras2 LB 943 Microplate Reader Terbium (Tb) Assay Setup Guide | Compatible | Tested |
| Excitation filter | 340x26 | 405x10 | 320x40 | 405x10 | |
| Emission 1 filter | 665xm7uv | 460m25 | 520xm10uv | 460m25 | |
| Emission 2 filter | 620xm10uv | 535m25 | 495xm10uv | 535m25 | |
| Dichronic mirror | Fixed | Fixed | Fixed | Fixed | |
| Note: filters for the Mithras² LB 943 use the notation: excitation filters as center wavelength x bandwidth (nm) and emission filters as center wavelength xm bandwidth or center wavelength m bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above. | |||||
Berthold Technologies Tristar² S LB 942 instrument configuration
| Assay technology | Adapta and LanthaScreen Eu | GeneBLAzer | LanthaScreen Tb | Predictor | Z'-LYTE |
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | Tristar2 S LB 942 Microplate Reader Europium (Eu) Assay Setup Guide | Compatible, not tested | Tristar2 S LB 942 Microplate Reader Terbium (Tb) Assay Setup Guide | Not compatible | Compatible, not tested |
| Excitation filter | 340x26 | 405/10 | 320x40 | 405/10 | |
| Emission 1 filter | 665xm7uv | 460/25 | 520xm10uv | 460/25 | |
| Emission 2 filter | 620xm10uv | 535/25 | 495xm10uv | 535/25 | |
| Dichronic mirror | Fixed | Fixed | Fixed | Fixed | |
| Excitation and Emission filters for the Tristar² S LB 942 use the notation: center wavelength/bandwidth (nm). Contact Berthold Technologies for information on ordering filters for the microplate readers listed above. | |||||
For Research Use Only. Not for use in diagnostic procedures.