Probing quaternary structure of protein complexes
Sequencing can also be performed using limiting proteolysis. In this approach, enzymes are introduced in a limited amount with the strategy that the protein stereochemistry dictates the digestion rather than the enzymes specificity. MS acquisition is done similar to bottom-up proteomics workflow. This approach can also be used to probe the quaternary structure of protein complexes.
Click image to enlarge
Featured products for limited proteolysis mass spectrometry
