DNA Analysis

Applied Biosystems GlobalFiler STR PCR amplification kits combine reduced amplification time with maximum discrimination power. As part of the only fully integrated and validated forensic workflow, this breakthrough 6-dye, 24-locus technology is designed to deliver superior lab performance so you can do more with less.

The GlobalFiler kit is also available with an Internal Quality Control system, or IQC, as part of a fully integrated and validated forensic workflow. The IQC system consists of two synthetic sequences with specific primers for each of the targets (IQCS and IQCL) and provides positive confirmation of sample amplification, but can also indicate adverse conditions with compromised amplification, such as the presence of PCR inhibitors. The IQC system, also used in our VeriFiler Plus and NGM Detect kits, provides additional confidence in genotyping results, and can help users distinguish, for example, between inhibited and degraded DNA samples.

VeriFiler Plus and VeriFiler Express PCR Amplification kits are designed to meet challenging forensic casework and databasing needs globally. Utilizing our 6-dye STR chemistry, the VeriFiler kits contain a total of 25 markers—23 autosomal STRs, including Penta D and Penta E, and two gender discrimination markers.

With 11 mini-STRs, VeriFiler Plus kits are specifically designed for challenging sample types: touch, inhibited, or degraded samples, with improved sensitivity and robustness to inhibition. In addition, an Internal Quality Control (IQC) system assesses for the presence of inhibitors in the sample to enable distinction between degraded and inhibited samples, which can help forensics analysts make better decisions on how to further process problematic samples. The IQC system also verifies PCR amplification.  Although optimized for casework samples, direct amplification of single-source reference samples using the VeriFiler Plus kit is also supported to enable laboratories process all sample types with one amplification kit.

The Applied Biosystems Yfiler Plus PCR Amplification Kit also leverages 6-Dye technology and contains 27 Y-STR markers that are valuable to use when evidence samples are difficult to resolve with traditional autosomal STR analysis, due to low amounts of male DNA present in a high background of female DNA.