StemPro hESC SFM – a fully-defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic stem cells (hESCs)
Tips for hESC Adaptation to StemPro hESC SFM
- From MEF conditioned media
- From other serum and feeder-free media
Serum- and Feeder-Free Medium
- Enables superior expansion of hESCs
- Maintains pluripotency and normal morphology / karyotype of hESCs
- Maintains differentiation capability of hESCs
- Provides gene expression profiles comparable to classical media
- Each lot is performance qualified using PCR
- No or little adaptation required from feeder cultures
- Eliminates variability associated with feeder-dependent cultures.
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| SSEA4 staining of hESCs grown in STEMPRO® hESC SFM | Tra-1-81 staining of hESCs grown in STEMPRO® hESC SFM | BG02 cells grown in STEMPRO® hESC SFM | BG02 cells grown in STEMPRO® hESC SFM |
Extensively tested
StemPro hESC SFM has been extensively tested and proven to maintain pluripotency in a growing list of hESC lines including:
| BG01 | BG02 | BG03 |
| H1 | H9 | BG01V |
| HUES9 | HUES6 | |
| CyT49 | Cyth203 | |
| Rhesus Monkey ES cells (line R366.4) | ||
Geltrex Matrix for hESC culture
Geltrex Reduced Growth Factor Basement Membrane Matrix is designed for use with STEMPRO hESC SFM as a matrix to support hESC growth. Geltrex has a consistently high lot to lot protein concentration and functional testing is performed on each lot.
Resources
STEMPRO hESC SFM Publication
About StemPro; hESC SFM
hESC Research Tools
For Research Use Only. Not for use in diagnostic procedures.