Salts and detergents used in protein sample preparation can adversely affect protein function and stability, interfering with downstream analyses. We offer a variety of resins and specialty devices in multiple formats for the efficient removal of salts and small molecule contaminants from protein samples. These products exhibit efficient and consistent performance and high sample recovery. In many cases, downstream analyses may also require that proteins and other biomolecules be at a higher concentration. To this end, we offer ultrafiltration protein concentrator devices that accommodate a wide range of sample volumes, remove excess liquids and buffers, and even exchange buffers. For removal of small molecules such as unconjugated fluorescent dyes, biotin, excess crosslinkers, and other reaction by-products, Pierce Dye and Biotin Removal Spin Columns can be used to achieve exceptional results.
Choose the right protein clean-up product for your experiments
| Dialysis Devices | Desalting Spin Columns | Concentrators | Dyes & Biotin Removal Columns | |
|---|---|---|---|---|
| Volume Range | 10 μL–250 mL | 2 μL–4 mL | 100 μL–100 mL | 50 μL–4 mL |
| Processing Time | 2–24 hrs | 5–10 min | 5–30 min* | 5–15 min |
| Applications | Desalting, buffer exchange, removal of unincorporated labels | Desalting, buffer exchange, removal of unincorporated labels | Sample concentration, buffer exchange | Removal of unincorporated dyes, biotin, crosslinkers, and reducing agents |
| Sample Type | Proteins, Cells | Proteins | Proteins | Purified proteins |
| Viscous Sample Compatibility | Yes | No | No | No |
| Sample Dilution | Possible | No | No | No |
| Gamma-Irradiated | Yes | No | No | No |
Make dialysis easy with the Slide-A-Lyzer G3 Dialysis Cassettes.
Stop fumbling with traditional dialysis tubing and try a more secure, user-friendly alternative. Slide-A-Lyzer G3 cassettes offer improved sample security, easier handling and sample access compared with traditional dialysis. This third-generation cassette is also improved to offer a more compact shape, easier opening cap, and significantly less plastic waste than previous generations.
Featured products for dialysis, desalting, protein concentrators, and small molecule removal
Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from samples containing proteins of interest that are larger than 7 kDa.
Simplify sample concentration, cleanup, and buffer exchange with Thermo Scientific Pierce Concentrators, which are available in five distinct molecular weight cutoffs (MWCOs) for volumes ranging between 100 μL–6 L.
Slide-A-Lyzer devices, cassettes, and flasks in a variety of formats and MWCOs, Pierce 96-well microdialysis plates, & SnakeSkin Dialysis Tubing.
Detergent removal resins in two formulations—optimized for high- or low-peptide concentrations—available in spin columns and 96-well spin filter plates.
Quickly remove salts and small molecules from protein solutions using Thermo Scientific Zeba Spin Columns, plates, and cartridges.
The Thermo Scientific Pierce RED system with easy-to-use inserts or preloaded plates for automation-compatible plasma protein binding assays.
Related categories
Mini- and microcentrifuges to help accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.
Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.
Detect and measure bacterial endotoxin levels using our portfolio of endotoxin assays, including the Pierce Rapid Gel Clot Endotoxin Kit and the Pierce Chromogenic Endotoxin Quantitation Kit. Reduce endotoxin levels up to 99% in less than one hour using our Pierce High Capacity Endotoxin Removal Spin Columns, with protein recovery ≥85%.
Resources
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