The Gibco™ CTS™ DynaCellect™ Magnetic Separation System enables users to easily scale from research to clinical and commercial manufacturing. This highly scalable system allows for 10 mL to 1 L of reaction volume (with an optimal density of 100 million to 10 billion target cells) for cell isolation. Since a continuous flow process is used for bead removal, the volume is potentially unlimited. Used with CTS Dynabeads for isolation of washed or unwashed cells, this system results in excellent isolation efficiency, purity, viability, cell activation, and target cell recovery.
High purity
Unwashed starting material
After isolation
Cell phenotype of starting material and enriched target cells after isolation. Purity of the target cells from unwashed starting material was ~52%. After isolation using CTS Dynabeads CD3/CD28 and the CTS DynaCellect Magnetic Separation System, the purity of target cell CD4 and CD8 (positive and negative) was ~97% as measured by flow cytometry. Bars represent mean of frequency of parent population gated on viable cells from four independent experiments.
Isolation efficiency and cell viability
Isolation efficiency
Cell viability
Strong isolation efficiency with no impact on cell viability. Using CTS Dynabeads CD3/CD28 and the CTS DynaCellect Magnetic Separation System, isolation efficiency was >86% (n = 4) and cell viability was maintained at >87%, as determined by flow cytometry.
Activation
Activation of isolated target cells is confirmed. Using CTS Dynabeads CD3/CD28 on Day 1, early activation marker CD69 is expressed on target cells and is downregulated by Day 7. Late activation marker CD25 is expressed on Day 1 and continues to be upregulated and expressed by Day 7. Expression profile adheres to expected target cell kinetics (n = 4).
Target cell recovery
Bead removal process achieves high recovery of cells. Recovery after CTS Dynabeads CD3/CD28 removal was >91% (n = 4). Recovery was assessed by cell counting before and after bead removal.
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