Analyzes the Tm, MW and extinction coefficient of a primer, and gives dilution instructions.
Online tools for analyzing primers, setting up reactions, and performing biochemical conversions & calculations.
Primer design and analysis
Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.
Restriction digestion tools
Performs double digestion with Thermo Scientific restriction enzymes.
Finds restriction enzymes either by name or recognition sequence.
Reaction setup calculators
PCR reaction setup with Thermo Scientific Phusion DNA Polymerases.
qPCR reaction setup with Thermo Scientific DyNAmo qPCR Kits.
Biochemical conversions
Calculates DNA and RNA concentrations based on measured OD260 values.
Analyzes qPCR amplification efficiency based on the slope of the standard curve.
Calculates copy numbers for DNA originating from different organisms.
PCR fidelity
Compare the error rate of Thermo Scientific Phusion High-Fidelity DNA Polymerase with other DNA polymerases.