Learn more in The Molecular Probes® Handbook
- Kits for Labeling Proteins and Nucleic Acids—Section 1.2
- Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3
- Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
- Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
- Coumarins, Pyrenes and Other Ultraviolet Light–Excitable Fluorophores—Section 1.7
- Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
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Table 1.3 Molecular Probes kits for protein and nucleic acid labeling.
| Kit Name | # Labelings | Kit Components | Features | |
|---|---|---|---|---|
| Kits for Labeling Proteins with Fluorescent Dyes | ||||
| APEX Antibody Labeling Kit | 5 labelings of 10–20 µg each of IgG antibody |
| APEX Antibody Labeling Kits utilize a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX antibody labeling tip. Any contaminants, including stabilizing proteins, are eluted through the tip prior to labeling with the amine-reactive fluorescent dye. The fluorescent IgG conjugate is ready to use in 2.5 hours, with ~15 minutes hands-on time. | |
| SiteClick R-PE Antibody Labeling Kit SiteClick Qdot Antibody Labeling Kits | 1 labeling of 100–125 µg of IgG antibody |
| The SiteClick system is a modular, click chemistry–mediated method for enzymatically labeling essentially any antibody on its heavy chain N-linked glycans—far from the antigen-binding domain. The SiteClick antibody labeling method prevents disruption of the antigen-binding domain that can occur with traditional amine- or thiol-reactive labeling reagents and eliminates the need to genetically engineer labeling sites into the antibody prior to modification. | |
| Alexa Fluor Antibody Labeling Kit Pacific Blue Antibody Labeling Kit Pacific Orange Antibody Labeling Kit | 5 labelings of ~100 µg each of carrier-free antibody samples |
| A buffered solution of the protein is added to one of the five vials of amine-reactive dye. The reactive dye has a succinimidyl ester (or tetrafluorophenyl ester) moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. The conjugate can be purified on the included size-exclusion spin columns. Labeling and purification can be completed in less than 2 hours. | |
| SAIVI Rapid Antibody Labeling Kit | 3 labelings of 0.5–3 mg each of carrier-free antibody solution |
| SAIVI Antibody Labeling Kits provide a convenient means to label antibodies with an optimal degree of labeling for in vivo imaging applications over a 6-fold antibody concentration range with no adjustments in reaction volume, dye concentration or antibody concentration necessary. Purification of the dye-labeled antibody is achieved with a simple protocol that can be completed in less than 10 minutes. These optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injection into animals. | |
| Alexa Fluor Microscale Protein Labeling Kit | Three 20–100 µg protein samples of a 12,000- to 150,000-dalton protein |
| Alexa Fluor Microscale Protein Labeling Kits provide a convenient means for labeling small amounts (20–100 µg) of purified protein with our superior Alexa Fluor dyes and purifying the resulting conjugate. Convenient spin columns are used to purify the labeled protein, with yields between 60 and 90% depending primarily on the molecular weight of the starting material. Labeling and purification can be completed in as little as 30 minutes. Microscale Protein Labeling Kits are also available for biotinylating proteins (see below). | |
| Easy-to-Use Protein Labeling Kit | Three ~1 mg protein samples of a 150,000-dalton protein, such as an IgG |
| A buffered solution of the protein is added to one of the three vials of the amine-reactive dye. The reactive dye has a succinimidyl ester (or tetrafluorophenyl ester) moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. Purification of the conjugate can be accomplished on the included gravity-feed size-exclusion columns. | |
| FluoReporter Protein Labeling Kit | 5 to 10 protein samples of 0.2–2 mg each in 200 µL volumes |
| The amount of dye necessary for the desired protein sample is calculated using the guidelines outlined in the kit protocol. The reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. Purification of the conjugate can be easily accomplished using the included spin columns. | |
| Zenon Antibody Labeling Kit | See Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3 | |||
| Kits for Labeling Proteins with Biotin or Dinitrophenyl (DNP) | ||||
| APEX Biotin- XX Antibody Labeling Kit | 5 labelings of 10–20 µg each of IgG antibody |
| APEX Antibody Labeling Kits utilize a solid-phase labeling technique; see complete description above. The biotinylated IgG conjugate is ready to use in 2.5 hours, with ~15 minutes hands-on time. | |
| Biotin-XX Microscale Protein Labeling Kit | Three 20–100 µg protein samples of a 12,000- to 150,000-dalton protein |
| The Biotin-XX Microscale Protein Labeling Kit provides a convenient means for biotinylating small amounts (20–100 µg) of purified protein. Convenient spin columns are used to purify the labeled protein with yields between 60 and 90%, depending primarily on the molecular weight of the starting material. Labeling and purification can be completed in as little as 30 minutes. For determining the degree of labeling, the FluoReporter Biotin Quantitation Assay Kit for proteins is available separately or in combination with the Biotin-XX Microscale Protein Labeling Kit. | |
| FluoReporter Mini-Biotin-XX Protein Labeling Kit | 5 biotinylation reactions of 0.1–3 mg each |
| The biotin-XX sulfosuccinimidyl ester (SSE) is water soluble and reacts with primary amines of proteins or other biomolecules to form stable biotin conjugates. The biotin-XX SSE has a 14-atom spacer that enhances the binding of biotin derivatives to avidin's relatively deep binding sites. Ready-to-use spin columns are included for purification of the biotinylated protein from excess reagents. | |
| FluoReporter Biotin-XX Protein Labeling Kit | 5 biotinylation reactions, each with 5–20 mg of protein |
| The biotin-XX succinimidyl ester (SE) reacts with primary amines of proteins or other biomolecules to form stable biotin conjugates. The biotin-XX SE has a 14-atom spacer that enhances the binding of biotin derivatives to avidin's relatively deep binding sites. A gel filtration column is provided for purifying the labeled proteins from excess biotin reagent. After purification, the degree of biotinylation can be estimated using the included avidin–biotin displacement assay. | |
| FluoReporter Biotin/DNP Protein Labeling Kit | 5 to 10 labeling reactions of 0.2–2 mg of protein each |
| The FluoReporter Biotin/DNP Protein Labeling Kit is similar to other FluoReporter Protein Labeling Kits, except that it contains DNP-X–biocytin-X succinimidyl ester as the reactive label. When proteins are labeled with this chromophoric biotin derivative, the degree of biotinylation can be readily assessed from the extinction coefficient of DNP (EC360 nm = 15,000 cm-1M-1). An additional feature of the conjugates labeled with DNP-X–biocytin-X succinimidyl ester is that they can be recognized by avidin derivatives (or anti-biotin antibodies) and by anti-DNP antibodies, enabling researchers to choose among several detection techniques suitable for fluorescence and electron microscopy. | |
| DSB-X Protein Labeling Kit | 5 protein conjugations of 0.5–3 mg each |
| DSB-X biotin succinimidyl ester, a derivative of desthiobiotin with an additional seven-atom spacer, reacts with amine groups of biomolecules to form stable amides. The DSB-X biotin conjugate can be detected with avidin or streptavidin derivatives. Binding is almost totally reversed by addition of free biotin at neutral pH and normal ionic strength. Materials are included for both small- and large-scale preparations. | |
| Kits for Labeling Nucleic Acids with Fluorescent Dyes | ||||
| ULYSIS Nucleic Acid Labeling Kit | 20 labelings of 1 µg DNA |
| The ULS reagent reacts with the N-7 position of guanine residues to provide a stable coordination complex between the nucleic acid and the fluorophore label. Separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished through a simple procedure using a spin column (not provided). | |
| ARES DNA Labeling Kit | 10 labelings of 1–5 µg DNA |
| In the first step, an amine-modified nucleotide, 5-(3-aminoallyl)-dUTP, is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using an amine-reactive fluorescent dye. The amine-modified DNA can be purified using the PureLink PCR Purification Kit (K3100-01). | |
| FISH Tag DNA Kit | See Labeling Oligonucleotides and Nucleic Acids—Section 8.2 | |||
| FISH Tag RNA Kit | See Labeling Oligonucleotides and Nucleic Acids—Section 8.2 | |||
| Oligonucleotide Amine Labeling Kit | 3 labelings of 50 µg each of an amine-modified oligonucleotide |
| The reactive dye used in the labeling has an amine-reactive succinimidyl ester moiety that reacts efficiently with an amine-modified oligonucleotide. Following the labeling reaction, the conjugate can be purified from the reaction mixture by preparative gel electrophoresis or reverse-phase HPLC. | |
For Research Use Only. Not for use in diagnostic procedures.