Applications Compendium for Sanger Sequencing and Fragment Analysis
An online collection of innovative Application Notes and Articles focused on Capillary Electrophoresis Workflows
Presented below is a collection of recent application notes and protocols describing selected applications utilizing Sanger sequencing and fragment analysis techniques on Applied Biosystems capillary electrophoresis genetic analyzers. Our customers and Applied Biosystems scientists have contributed to the articles in this collection. We hope that reading about their work will help guide your current research and spark ideas for other innovative applications.
The 3500 Series Genetic Analyzer
- Fungal DNA sequencing from laser capture microdissection samples
- SNP Gentyping Using SNaPshot® Multiplex Kit and 3500 Series Genetic Analyzer
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- ISSR Genotyping of Endangered Plants Using an Optimized Workflow
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
- Uniparental disomy (UPD) analysis of chromosome 15 (3.2MB)
- High coverage gene expression profiling on the Applied Biosystems® 3500xL Genetic Analyzer (1.6MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
Cell and Gene Therapy
BAC Fingerprinting
- Sizing of Large DNA Fragments Generated by BAC Fingerprinting on Capillary Electrophoresis System
- BAC Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer
Cancer
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
Copy Number Analysis
- Gene Copy Number analysis using semi-quantitative multiplex PCR-based assay on capillary electrophoresis systems
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
Epigenetics and Methylation
- Three Optimized Workflows for CpG Island Methylation Profiling
- Methylation Analysis by Bisulfite Sequencing: Chemistry, Products and Protocols from Applied Biosystems
- Application Note: MethylSeq Direct workflow: a fast method for DNA methylation analysis.
Genetic Disorders
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
Gene Expression Profiling
Genotyping
- SNP Gentyping Using SNaPshot® Multiplex Kit and 3500 Series Genetic Analyzer
- ISSR Genotyping of Endangered Plants Using an Optimized Workflow
Microsatellite (STR) Analysis
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- Uniparental disomy (UPD) analysis of chromosome 15 (3.2 MB)
Mutation Detection
- Equivalent Performance of the Applied Biosystems™ 3500 and SeqStudio™ Flex Genetic Analyzer CE Systems for AmplideX® Gene Variant Analysis
- Detection and Quantification of Sequence Variants from Sanger Sequencing Traces
- Genotyping KRAS and BRAF Mutations in Paraffin-embedded Samples for Cancer Research
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Single Strand Conformation Polymorphism (SSCP) Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- Development of a resequencing workflow for variant analysis in the APC gene (1MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
- BigDye® sequencing reagents for rapid detection of mutations in non-small cell lung carcinoma research samples
Plants, Animals, and Agriculture
Resequencing
- ABRF 2011 - A New Sequencing Primer and Workflow Increase 5' Resolution and Throughput on HLA Sequencing
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
- Development of a resequencing workflow for variant analysis in the APC gene (1MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
Review our comprehensive list of publications describing Sanger sequencing and fragment analysis applications used in conjunction with next-generation sequencing.
The Sanger Sequencing Publication List
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The 3500 Series Genetic Analyzer
- Fungal DNA sequencing from laser capture microdissection samples
- SNP Gentyping Using SNaPshot® Multiplex Kit and 3500 Series Genetic Analyzer
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- ISSR Genotyping of Endangered Plants Using an Optimized Workflow
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
- Uniparental disomy (UPD) analysis of chromosome 15 (3.2MB)
- High coverage gene expression profiling on the Applied Biosystems® 3500xL Genetic Analyzer (1.6MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
Cell and Gene Therapy
BAC Fingerprinting
- Sizing of Large DNA Fragments Generated by BAC Fingerprinting on Capillary Electrophoresis System
- BAC Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer
Cancer
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
Copy Number Analysis
- Gene Copy Number analysis using semi-quantitative multiplex PCR-based assay on capillary electrophoresis systems
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
Epigenetics and Methylation
- Three Optimized Workflows for CpG Island Methylation Profiling
- Methylation Analysis by Bisulfite Sequencing: Chemistry, Products and Protocols from Applied Biosystems
- Application Note: MethylSeq Direct workflow: a fast method for DNA methylation analysis.
Genetic Disorders
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
Gene Expression Profiling
Genotyping
- SNP Gentyping Using SNaPshot® Multiplex Kit and 3500 Series Genetic Analyzer
- ISSR Genotyping of Endangered Plants Using an Optimized Workflow
Microsatellite (STR) Analysis
- Aneuploidy Detection by QF-PCR of STR Markers on the Applied Biosystems 3500xL Genetic Analyzer
- Uniparental disomy (UPD) analysis of chromosome 15 (3.2 MB)
Mutation Detection
- Equivalent Performance of the Applied Biosystems™ 3500 and SeqStudio™ Flex Genetic Analyzer CE Systems for AmplideX® Gene Variant Analysis
- Detection and Quantification of Sequence Variants from Sanger Sequencing Traces
- Genotyping KRAS and BRAF Mutations in Paraffin-embedded Samples for Cancer Research
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Single Strand Conformation Polymorphism (SSCP) Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- Development of a resequencing workflow for variant analysis in the APC gene (1MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
- BigDye® sequencing reagents for rapid detection of mutations in non-small cell lung carcinoma research samples
Plants, Animals, and Agriculture
Resequencing
- ABRF 2011 - A New Sequencing Primer and Workflow Increase 5' Resolution and Throughput on HLA Sequencing
- Resequencing Workflow for MLH1 and MSH2 Variant Analysis Application Note
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- Development of an Interlaboratory-Verified Sequencing Workflow for KRAS Variant Identification
- DNA Resequencing Analysis of X-linked Variants Using the Applied Biosystems 3500xL Genetic Analyzer
- Development of a resequencing workflow for variant analysis in the APC gene (1MB)
- BigDye® Direct Cycle Sequencing Kit simplifies workflow for characterizing melanoma mutations in research samples
Review our comprehensive list of publications describing Sanger sequencing and fragment analysis applications used in conjunction with next-generation sequencing.
The Sanger Sequencing Publication List
Showing of | Show All
Sort by:
For Research Use Only. Not for use in diagnostic procedures.