NovaBright beta-galactosidase reporter gene assay for mammalian or yeast cells combines direct cell lysis with the rapid ultrasensitive detection of beta-galactosidase reporter enzyme in a homogenous assay format. The kit uses the combination of Galacton-Star substrate and Sapphire-II enhancer to give sensitivity and dynamic range unmatched by colorimetric methods. The kit is formatted with two alternative reaction buffers for the lysis of mammalian or yeast cells.
NovaBright β-galactosidase Reporter Gene Assays
NovaBright β-galactosidase Reporter Gene Assays are available for yeast or mammalian cells and combine direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase.
- Homogeneous assay is automation-friendly
- Persistent glow readout remains constant for 45 to 90 minutes
- Sensitivity from picogram to nanogram amounts of reporter
Sensitivity of NovaBright β-galactosidase Reporter Gene Assay Detection. NovaBright β-galactosidase Reporter Gene Assays were performed with the indicated amount of purified β-galactosidase diluted in PBS. Assays were measured on a microplate luminometer.
Workflow
More NovaBright Chemiluminescent Reporter Gene Assays
- Learn more about NovaBright Chemiluminescent SEAP Reporter Gene Assays
For Research Use Only. Not for use in diagnostic procedures.