Standardize your SARS-CoV-2 RNA extraction method
Achieve faster, more consistent purification of SARS-CoV-2 viral RNA from your samples with Applied Biosystems viral RNA extraction kits. Our kits allow you to efficiently extract viral RNA from sources with a low abundance of nucleic acid. Purified viral RNA is compatible with most downstream assays, including RT-PCR, RT-qPCR, and NGS.
Partner with Thermo Fisher Scientific to go from sample to results. Our technical teams provide comprehensive onsite or remote support so that you can keep the lab up and running.
Viral RNA extraction kits optimized for SARS-CoV-2
MagMAX magnetic bead-based kits offer flexible, highly efficient methods for purifying nucleic acids. The following products are specifically recommended for SARS-CoV-2 viral RNA extraction.
MagMAX Microbiome Ultra Nucleic Acid Isolation Kits | MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I) | MagMAX Bulk Reagents for Viral RNA extraction | MagMAX Prime Viral/Pathogen NA Isolation kit  | |
| Sample types | Stool, swabs, transport media, culture media, urine, saliva, and soil | Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and saliva | Swabs in transport media (respiratory), saliva, urine | |
| Best for | Solid samples and wastewater | Large sample volumes | Highly customized protocols | Running various sample types on a single plate |
| Cat. No. | A42357 (100 preps), with bead plate A42358 (100 preps), with bead plate | A48310 (up to 2,000 preps/kit) A42352 (up to 200 preps/kit) | A42359 (binding solution) A42360 (wash solution) A42362 (binding beads) A42363 (proteinase K) A42364 (elution buffer) | A58145 (up to 600 preps/kit) A58147 (binding beats) A58148 (elution buffer) A58149 (wash I solution) A58150 (binding solution) A58151 (proteinase K) A58152 (proteinase K dye) |
Application Note: Detection of SARS-CoV-2 in fecal samples and wastewater
Instruments for SARS-CoV-2 viral RNA extraction
KingFisher magnetic bead processing instruments are ideal for automating viral RNA extraction protocols. Experience consistent extraction and purification of viral RNA from 6–96 samples in parallel with KingFisher automated sample purification systems
Microplates and tip combs for KingFisher magnetic processing instruments
Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.
How to use MagMAX kits and Kingfisher instruments for SARS-CoV-2 viral RNA extraction
See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.
- How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System
- How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System
- How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation
- How to use a Multidrop Combi Reagent Dispenser to prepare KingFisher plates for SARS-CoV-2 sample preparation
- How to use a Spectrum DNA SDNA-1000 saliva collection kit
- How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit
- How to use a 50 mL conical tube for saliva collection
- How to use a GeneFIX Saliva DNA/RNA collection device
How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System
How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System
How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation
Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.
How to use a Spectrum DNA SDNA-1000 saliva collection kit
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a 50 mL conical tube for saliva collection
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a GeneFIX Saliva DNA/RNA collection device
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.
Kingfisher protocol files for SARS-CoV-2 testing
KingFisher Flex protocols for viral RNA extraction
The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).
| Protocol type | Standard wash (3 wash steps) | Reduced wash (2 wash steps) |
|---|---|---|
| 200 μL script | MVP_Flex_200ul.bdz | MVP_2Wash_200_Flex.bdz |
| 400 μL script | MVP_Flex_400ul.bdz | MVP_2Wash_400_Flex.bdz |
KingFisher Flex protocol for extraction of viral RNA from saliva
The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).
Viral RNA extraction kits optimized for SARS-CoV-2
MagMAX magnetic bead-based kits offer flexible, highly efficient methods for purifying nucleic acids. The following products are specifically recommended for SARS-CoV-2 viral RNA extraction.
| MagMAX Microbiome Ultra Nucleic Acid Isolation Kits | MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I) | MagMAX Bulk Reagents for Viral RNA extraction | MagMAX Prime Viral/Pathogen NA Isolation kit | |
| Sample types | Stool, swabs, transport media, culture media, urine, saliva, and soil | Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and saliva | Swabs in transport media (respiratory), saliva, urine | |
| Best for | Solid samples and wastewater | Large sample volumes | Highly customized protocols | Running various sample types on a single plate |
| Cat. No. | A42357 (100 preps), with bead plate A42358 (100 preps), with bead plate | A48310 (up to 2,000 preps/kit) A42352 (up to 200 preps/kit) | A42359 (binding solution) A42360 (wash solution) A42362 (binding beads) A42363 (proteinase K) A42364 (elution buffer) | A58145 (up to 600 preps/kit) A58147 (binding beats) A58148 (elution buffer) A58149 (wash I solution) A58150 (binding solution) A58151 (proteinase K) A58152 (proteinase K dye) |
Application Note: Detection of SARS-CoV-2 in fecal samples and wastewater
Instruments for SARS-CoV-2 viral RNA extraction
KingFisher magnetic bead processing instruments are ideal for automating viral RNA extraction protocols. Experience consistent extraction and purification of viral RNA from 6–96 samples in parallel with KingFisher automated sample purification systems
Microplates and tip combs for KingFisher magnetic processing instruments
Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.
How to use MagMAX kits and Kingfisher instruments for SARS-CoV-2 viral RNA extraction
See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.
- How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System
- How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System
- How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation
- How to use a Multidrop Combi Reagent Dispenser to prepare KingFisher plates for SARS-CoV-2 sample preparation
- How to use a Spectrum DNA SDNA-1000 saliva collection kit
- How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit
- How to use a 50 mL conical tube for saliva collection
- How to use a GeneFIX Saliva DNA/RNA collection device
How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System
How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System
How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation
Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.
How to use a Spectrum DNA SDNA-1000 saliva collection kit
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a 50 mL conical tube for saliva collection
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.
How to use a GeneFIX Saliva DNA/RNA collection device
Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.
Kingfisher protocol files for SARS-CoV-2 testing
KingFisher Flex protocols for viral RNA extraction
The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).
| Protocol type | Standard wash (3 wash steps) | Reduced wash (2 wash steps) |
|---|---|---|
| 200 μL script | MVP_Flex_200ul.bdz | MVP_2Wash_200_Flex.bdz |
| 400 μL script | MVP_Flex_400ul.bdz | MVP_2Wash_400_Flex.bdz |
KingFisher Flex protocol for extraction of viral RNA from saliva
The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).
SARS-CoV-2 Research Solutions
From viral research and epidemiological studies to potential vaccine development.
Learn more ›Resources for coronavirus RNA extraction
For Research Use Only. Not for use in diagnostic procedures.