Flow cytometry is a powerful tool in the study of many areas of biology. In this subtopic section you will find educational assets that include application notes, selection guides, videos, webinars, tutorials and other tools related to flow cytometry and its use in immunology, immuno-oncology, cancer, and stem cell research.
Areas of biology features
Research overview
Immuno-oncology research using flow cytometry
Learn how flow cytometry analysis is used in immuno-oncology research areas, including investigations into the tumor microenvironment, immune checkpoint analysis, and CAR T characterization.
BioProbes article
A comprehensive resource for state-of-the-art flow cytometry methods
This article provides a review of recently published and extensive guidelines for the use of flow cytometry and cell sorting in immunological studies, as well as an infographic giving tips for key data controls.
Areas of biology subtopics
Areas of biology learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | Attune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research |
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| Application note (2015) | Live/dead algal analysis with the Attune NxT Flow Cytometer | algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability |
| BioProbes article (2018) | Immuno-oncology: Advances in Basic Research and Translational Medicine. With a focus on immune checkpoint inhibitors and T cell immunotherapy | antibodies, ELISA, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis (HCA), immunohistochemistry (IHC), immunology, immuno-oncology, immunophenotyping, Luminex, ProCartaPlex, spheroids |
| BioProbes article (2018) | Flow cytometric assay for simultaneous assessment of HIV RNA and Gag protein. Single-cell characterization of viral translation-competent reservoirs in HIV-infected individuals | antibodies and proteins, flow cytometry, HIV, immunology, in situ hybridization, PrimeFlow, protein expression, RNA detection |
| BioProbes article (2018) | A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studies | antibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability |
| BioProbes article (2017) | Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays | antibodies, cancer, flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC) |
| BioProbes article (2017) | Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometer | apoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability |
| BioProbes article (2016) | High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometry | Attune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability |
| BioProbes article (2016) | A complete suite of reagents, from cas9 delivery tools to cell function Assays—The CRISPR-cas9 system for genome editing | CRISPR, flow cytometry assays, fluorescence, fluorescence microscopy/fluorescence imaging, high content analysis, western blotting |
| BioProbes article (2016) | Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research |
| BioProbes article (2015) | Tools and strategies for rare-event detection using flow cytometry | Attune NxT, flow cytometer, fluorescence, rare cell detection, rare event detection |
| BioProbes article (2009) | A flow cytometry–based method for isolating neural stem cells | Alexa Fluor, cell isolation, flow cytometry, fluorescent dyes, neuroscience, stem cell research |
| BioProbes article (2008) | Putting regulatory T cells to work—Isolate and expand CD4+ CD25+ Treg cells using Dynabeads magnetic separation technology | cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, reagents |
| BioProbes article (2007) | The latest in stem cell research | antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research |
| Guided learning | T cell stimulation and proliferation eLearning course | antibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells |
| Immunology information | Adaptive immunity overview | immunology |
| Immunology information | B cell activation functional assay | immunology |
| Immunology information | Costimulatory and inhibitory interactions control T cell response | immunology, stimulation, T cells |
| Immunology information | Foxp3 overview | flow cytometry, Foxp3, intracellular staining |
| Immunology information | Innate immunity overview | immunology |
| Immunology information | Innate lymphoid cells overview | immunology |
| Immunology information | Microbiome (Microbiota) overview | immunology |
| Immunology information | Natural killer cells overview | immunology |
| Immunology information | T cell activation functional assay | immunology |
| Immunology information | T follicular helper differentiation functional assay | immunology |
| Immunology information | T reg differentiation functional assay | immunology |
| Immunology information | T regulatory cells overview | immunology |
| Immunology information | Th1 differentiation functional assay | immunology |
| Immunology information | Th17 cells overview | immunology |
| Immunology information | Th17 differentiation functional assay | immunology |
| Immunology information | Th2 differentiation functional assay | immunology |
| Immunology information | Th22 differentiation functional assay | immunology |
| Immunology information | Th9 differentiation functional assay | immunology |
| Immunology information | Toll-like receptor overview | immunology |
| Molecular Probes Handbook | MHC tetramer technology—Note 7.2 | antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells |
| Pathways | Adaptive immunity pathways | immunology |
| Pathways | Innate immunity pathways | immunology |
| Pathways | Inflammatory response pathway | immunology |
| Pathways | Interferon (IFN) cell signaling pathway | immunology |
| Pathways | T-cell receptor (TCR) cell signaling pathway | immunology |
| Pathways | Toll-like receptor (TLR) cell signaling pathway | immunology |
| Scientific poster (2018) | Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage | Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research |
| Scientific poster (2018) | Modular, defined assembly of antibody-based constructs using click chemistry | Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids |
| Scientific poster (2018) | Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia | antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research |
| Scientific poster (2018) | Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia | Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry |
| Scientific poster (2018) | Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma | Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions |
| Scientific poster (2018) | Fluorescent hypoxia sensor for flow cytometry | apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology |
| Scientific poster (2011) | The next step in the analysis of lymphocyte proliferation | Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents |
| Scientific poster (2010) | A comparison of three techniques to induce efficient ex vivo T cell expansion | Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Selection guide | Cytokine bioassays guide | cytokines, flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Human CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Mouse CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| CYToMORE lecture video (2016) | Assessing immunological aging using flow cytometry Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore | flow cytometry assays, immunology |
| CYToMORE lecture video (2016) | Cancer stem cells and multi-drug resistance by flow cytometry Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain | cancer, flow cytometry, multidrug resistance (MDR), stem cell research |
| CYToMORE lecture video (2016) | Cytometric analysis of cytokines and transcription factors Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany | cell signaling, flow cytometry, immunophenotyping |
| CYToMORE lecture video (2016) | Cytometry of immunity Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany | flow cytometry, immunology |
| CYToMORE lecture video (2016) | Cytomic analysis of oxidative stress Presented by J. Enrique O’Connor, University of Valencia, Spain | flow cytometry, oxidative stress |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Host-microbe interaction at mucosal surfaces Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy | flow cytometry, immunology, microbiology |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | mass cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Microenvironment cytometry Presented by Paul Smith, Cardiff University, UK | cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery |
| CYToMORE lecture video (2016) | Rare cells analysis: from bench to clinics Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy | Attune NxT, flow cytometer, rare cell detection, rare event detection |
| Technology video | The Two Worlds of Cell Separation | cell isolation, immunology |
| Webinar | The Complex Pharmacology of T-Cell CARs In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies. | flow cytometry, immuno-oncology |
| Webinar | Flow cytometry in microbiological research In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods. | microbiology, multicolor flow cytometry, viability |