Find comprehensive detection solutions designed for your western blotting success
Quick visualization, precise quantitation, single-probe detection or multiplexing — we offer a range of reagents, kits and instruments for western blot detection and analysis.
Western Blot Detection Reagents
Optimized buffers and reagents for every step of the western blot process.
Western Blot Automated Processing
Reproducible systems for western blot processing.
Western Blot Imaging and Analysis
Easy-to-use western blot and gel documentation instruments and software.
Manual vs. automated western blot processing
Probing a western blot prior to data visualization involves a series of manual steps, many of which are individually short but collectively require significant hands-on time. We offer instruments to automate some of these tasks, significantly decreasing hands-on time.
Manual and automated procedures share three essential steps: blocking the membrane, probing with primary and secondary antibodies, and washing the membrane. The manual western blot processing workflow requires more than 10 hands-on steps and at least 4 hours before final detection can occur. Automated western blot processing using the Invitrogen Bandmate Automated Western Blot Processor or Invitrogen iBind or iBind Flex Western Device requires only a 15–30 minute setup time and no additional hands-on steps before you can move to final detection.
| iBind Western Device | Bandmate Automated Western Blot Processor | |
|---|---|---|
 |  | |
| Technology | Sequential lateral flow | Programmable rocking system |
| Capacity | 1 mini blot (iBind) or up to 2 mini or 1 midi or 6 strips blots (iBind Flex) | Up to 4 mini blots or up to 2 midi blots |
| Required consumables | iBind Cards and iBind Solution | Reagent tubes |
| Antibody recovery | No | Yes, Primary and Secondary |
| Programmable | No | Yes, up to 9 programs |
For Research Use Only. Not for use in diagnostic procedures.