I am having problems with...
 | Your curves are sigmoidal or amplification occurs later than you expected. | |
 | You have an amplification product in your no template control (NTC). | |
 | You have no detectable PCR product. | |
 | If the slope of your standard curve is below -3.6, you have poor PCR efficiency. | |
 | Normal Amplification Curve Example Your amplification curve should look something like this |
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