Find a master mix for your real-time qPCR application
For ease of selection, our real-time qPCR master mixes are organized into two general categories: qPCR master mixes (DNA input) and one-step RT-qPCR master mixes (RNA input).
Real-time qPCR master mixes
1-step RT-qPCR master mixes (RNA input) |
qPCR master mixes (DNA input) |
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Recommended applications |
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1-step gene expression Pathogen detection |
1-step gene expression | DNA microbial detection |
SNP genotyping Copy number variation |
2-step gene expression |
Target/input |
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| RNA | RNA | DNA |
gDNA |
cDNA |
Concentration |
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| 4X | 4X | 2X |
2X |
2X |
Multiplexing and passive reference dye |
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| Available in two versions: With ROX (up to 5 targets) No ROX (up to 6 targets) |
Available in three versions: With ROX (up to 5 targets) No ROX (up to 6 targets) With Mustang purple (>3 targets) |
Available in two versions: |
Available in two versions: |
With ROX (up to 3 targets) |
Reverse transcriptase |
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| Thermostable MMLV RT included | Thermostable MMLV RT included | N/A |
N/A |
Purchase RT separately and use before qPCR |
Cycling mode/run time |
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| Standard: ~110 min Fast: ~60 min |
Standard: ~110 min Fast: ~60 min |
Standard: ~120 min |
Standard: ~120 min |
Standard: ~50 min |
Carryover contamination |
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| UNG compatible (UNG not included) | With UNG | With UNG |
With UNG |
With UNG |
| Order now | Order now | Order now | ||
TaqPath master mixes are general purpose reagents labeled ‘For Laboratory Use.’