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Configurations with up to 9 lasers and 60 detectors

 

Configurations offering both spectral analysis and conventional compensation range from 5 to 9 lasers and up to 60 detectors (55 fluorescence and 2-5 scatter parameters), while configurations offering only traditional compensation come with 4 or 5 lasers and up to 35 detectors (30 fluorescence plus 2-5 scatter parameters). Other than the laser and detector configuration, all instrument models share the same specifications.

Configurations

Configurations offering both spectral analysis and conventional compensation

Lasers Number of fluorescence detection channels for included lasers Scatter detectors Total detection channels Cat. No.
UV
349 nm		 Violet
405 nm		 Blue violet
445 nm		 Blue
488 nm		 Green
532 nm		 Yellow green
561 nm		 Red
594 nm		 Far red
640 nm		 Near-
IR
785 nm
5 12 12   7   12   5   5 53 PL00302
6 12 12   7   12   5 3 5 56 PL00301
6 12 12 4 7   12   5   5 57 PL00300
7 12 12 4 7   12   5 3 5 60 PL00299
9 12 12 4 7 12 5 3 5 60 PL00285*

*532 nm and 594 nm lasers are not used for spectral analysis, but all fluorescence detectors are used.

Configurations offering only conventional compensation

Lasers Number of fluorescence detection channels for included lasers Scatter detectors Total detection channels Cat. No.
UV
349 nm		 Violet
405 nm		 Blue violet
445 nm		 Blue
488 nm		 Green
532 nm		 Yellow green
561 nm		 Red
594 nm		 Far red
640 nm		 Near-
IR
785 nm
4 7   7   7   4   2 27 PL00304
5 7 7   5   7   4   5 35 PL00303
4   7   7   7   4   3 28 PL00370

Specifications

Optics

Excitation lasers 349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm,  594 nm, 640 nm, 785 nm
Optical power Free space delivery of 349 nm (100 mW), 405 nm (100 mW), 445nm (200 mW), 488 nm (125 mW), 532 nm (100 mW), 561 nm (100 mW), 594 nm (100 mW), 640 nm (100 mW), 785 nm (100 mW)
Beam alignment Fixed, 7 spatially separated pinholes
Detection parameters up to 55 fluorescence + 5 scatter
Scatter parameters Standard FSC and SSC 488 nm; small particle FSC 405 nm; depolarized FSC and SSC 488nm
Scatter resolution <0.2 µm scatter resolution from background with small particle detector
Pulse measurement Simultaneous measured peak, area, and width for sample input and output of every channel
Fluorescence sensitivity <100 MESF for FITC, PE, APC

Fluidics

Nozzles 70, 100, 120, and 150 µm ceramic nozzle tips with adjustable pressure settings
Sample input tube types 1.5 mL, 5 mL, and 15 mL
Temperature control 4°C-37°C for input and output of all media types
Agitation Automatic based on input tube type

Sorting

Event rate >100,000 eps acquisition with all 60 parameters
Sorting speed >70,000 eps with all parameters enabled*
Sort devices Up to 6-way sorting; configurable tube holders include 1.5 mL, 5 mL, 15 mL, and 50 mL tubes. Microwell plates with a standard footprint of up to 1,536 wells can be loaded directly for straight-down sorting; 4-way sorting into 96-well plates; 4-way sorting into 384 well plates. Temperature control from 4°C to 37°C for all sorting media. 


* When running at 70,000 eps, users may see a decrease in efficiency depending on experimental conditions and sort criteria. Sort speeds should always be optimized for specific experiments.

Biosafety

Compliance testing The Bigfoot Spectral Cell Sorter biosafety enclosure provides personnel and product protection similar to a Class II BSC. Test procedures and criteria laid out within NSF49 and EN12469 standards can be utilized to demonstrate performance.

Other

Dimensions 99 cm W x 99 cm D x 170 cm H (39 in W x 39 in D x 67 in H)
Fluidics cart None
Weight 1433 lbs (650kg)
Monitor 32-inch, 4k
Power supply 100-240VAC 50/60Hz
Power consumption 750W max (instrument, PC and monitor)
Relative humidity 20-60% RH

For Research Use Only. Not for use in diagnostic procedures.

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