Figure 1. Examples of available applications from the HCS Studio Cell Analysis Software.
Intuitive software, powerful high content cell analysis
Powerful image analysis tools, intuitive interfaces and EurekaScan™ Finder feature significantly reduce instrument runtime by capturing rare events at high magnification. The Thermo Scientific HCS Studio Cell Analysis Software expertly balances flexibility and ease-of-use using intelligent design resulting in meaningful dynamic data.
Features
- 'On-the-fly' real-time analysis, enabling fast results, simultaneous image acquisition and data analyses.
- Backtrack each event to perform analysis at the single-cell level and minimize artifacts in the data.
- Ranking assay parameters based on Z-prime values helps give you confidence for robust assay performance and allows you to select your top performing assays.
- Guided workflows with user-friendly visual feedback facilitates easy assay protocol setup, image acquisition, analysis, and scanning.
Optimized pre-established assays
Users of HCS Studio Cell Analysis Software start with more than 30 pre-established assays (Figure 1) that can be optimized to cell line and particular phenotype. Driven by intuitive icons, you can simply choose the assay and magnification of the optimized protocol, confirm settings, and begin the scan. We’ve done the work, so you don’t have to.
Build your own assay
Create your own assay from scratch using the flexible tools and design of HCS Studio Cell Analysis Software. HCS Studio Cell Analysis Software scales seamlessly, providing a host of algorithmic, visualization, and online help tools that reduce the complexity and time investment of developing assays. With instant feedback you can control hundreds of options including:
- Background correction
- Object segmentation
- Spot/granular detection
- Region of interests
- Sensitivity of detection
- Projected image format
- Contrast of images
- Phenotypic gating
Save time and effort with improved high-content screening
What is EurekaScan™ Finder?
- Thermo Scientific EurekaScan™ Finder is a new feature for HCS Studio software that significantly reduces your instrument runtime (CellInsight CX7 LED and CellInsight CX7 LZR) and analysis efforts by automating the detection and subsequent capture of events at higher magnification.
Why is there a need for EurekaScan™ for high-content screening?
- Without EurekaScan™ Finder, if the well is sparsely populated or if you need to image rare events at high magnification, scan times are significantly enhanced due to the instrument performing autofocus and imaging on the blank fields.
- Also, typical analysis times are prolonged as you need to review and discard a large number of irrelevant images.
The solution is EurekaScan™ Finder
- With EurekaScan™ Finder objects/events recognized at lower magnification during the first scan are automatically used to drive the scan area of the high magnification pass(es), thus saving time and effort.
- EurekaScan™ Finder can also handle more than two passes (double-pass high-content screening) for those scenarios that could benefit from three or more passes, such as identifying non-sparse fields at low magnification, finding rare events at higher magnification, and then evaluation of rare events at same or higher magnification.
Click image to enlarge
Figure 2. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X screen alone without EurekaScan™ Feature. All images acquired and analyzed. Data provided from Dr. Chi Yun at New York University.
Figure 2. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X screen alone without EurekaScan™ Feature. All images acquired and analyzed. Data provided from Dr. Chi Yun at New York University.
Click image to enlarge
Figure 3. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X EurekaScan™ screen. The EurekaScan™ Feature auto-selects the area of interest for imaging.
*EurekaScan™ Finder utilization produced a 14.6-fold improvement in screening time compared to conventional Non-EurekaScan Finder 10X alone. Additional efficiencies were captured when evaluating the file size of the two screens, with EurekaScan™ Feature exhibiting 15-fold improvement in memory usage to complete the screen. Data provided from Dr. Chi Yun at New York University.
Figure 3. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X EurekaScan™ screen. The EurekaScan™ Feature auto-selects the area of interest for imaging.
*EurekaScan™ Finder utilization produced a 14.6-fold improvement in screening time compared to conventional Non-EurekaScan Finder 10X alone. Additional efficiencies were captured when evaluating the file size of the two screens, with EurekaScan™ Feature exhibiting 15-fold improvement in memory usage to complete the screen. Data provided from Dr. Chi Yun at New York University.
Click image to enlarge
Figure 4. HCS Studio EurekaScan™ Feature results. During the 10X screen, both widefield and 70-micron pinhole confocal images were acquired using the 405 lazer. Data provided from Dr. Chi Yun at New York University.
Flexible with various sample types
EurekaScan™ Finder offers two different approaches based on the type of sample and user-stipulated criteria that will inform high-content screening:
Scenario I: when studying tissues
Click image to enlarge
Scenario I: In this scenario (tissue), the selected objects in first pass are bigger than the second pass’s field of view (case A), or multiple objects appear in the second pass’s field of view (case B).
In which case, the second pass will utilize a uniformly spaced lattice pattern to define the second pass’s field locations.
Scenario II: when studying spheroids, rare-events
Click image to enlarge
Scenario II: In this scenario (spheroids, rare events), the selected objects in the first pass are smaller than the second pass’s field of view and are spaced apart such that multiple objects are unlikely to be in the same field of view.
In which case, the second pass will center the field of views around each object.
Note: The software will automatically choose whether to “center-on-object” or use “lattice pattern” based upon each the selected objects average size and density, on a per-well basis.
Smart selection for high-content imaging and screening
- With the EurekaScan™ Feature applied, specimens including spheroids and tissues may be identified during “seek” operations at low magnification and once “found,” efficiently scanned at higher magnifications for optimal resolution.
- The EurekaScan™ Feature was designed to enable multiple pass scans, such as identifying samples at low magnification across large surface areas, capturing samples at intermediate magnification, and evaluating for rare events or improved resolution at higher magnifications.
- By employing the EurekaScan™ Feature for high-magnification scans, you can experience efficiencies in total scan times and corresponding file memory consumption.
- Moreover, object identification and verification parameters are applied in real time during EurekaScan™ Feature operation, so scientists have more confidence that the samples being adaptively examined are relevant to their research.
- Each pass can have its own unique analysis and identification process based on user-stipulated criteria. This includes different variables such as objectives, channels, different z-stacks, or even kinetics.
- Further acquisition time can be shortened by locking the same the reference position used for the z-stack acquisition during first pass and then using it for subsequent passes.
Click image to enlarge
Figure 5. A 4X scan of a single well of a 24-well plate containing brain organoids stained with tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) was performed. Brightfield was used for object selection for the second scan. The second pass is 20X, brightfield, widefield (WF) tyrosine hydroxylase (Alexa Fluor 488), WF neurofilament (Alexa Fluor 568) and confocal (CF) tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) performed using CellInsight CX7 LZR high content.
Figure 5. A 4X scan of a single well of a 24-well plate containing brain organoids stained with tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) was performed. Brightfield was used for object selection for the second scan. The second pass is 20X, brightfield, widefield (WF) tyrosine hydroxylase (Alexa Fluor 488), WF neurofilament (Alexa Fluor 568) and confocal (CF) tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) performed using CellInsight CX7 LZR high content.
Optimized pre-established assays
Users of HCS Studio Cell Analysis Software start with more than 30 pre-established assays (Figure 1) that can be optimized to cell line and particular phenotype. Driven by intuitive icons, you can simply choose the assay and magnification of the optimized protocol, confirm settings, and begin the scan. We’ve done the work, so you don’t have to.
Figure 1. Examples of available applications from the HCS Studio Cell Analysis Software.
Build your own assay
Create your own assay from scratch using the flexible tools and design of HCS Studio Cell Analysis Software. HCS Studio Cell Analysis Software scales seamlessly, providing a host of algorithmic, visualization, and online help tools that reduce the complexity and time investment of developing assays. With instant feedback you can control hundreds of options including:
- Background correction
- Object segmentation
- Spot/granular detection
- Region of interests
- Sensitivity of detection
- Projected image format
- Contrast of images
- Phenotypic gating
Save time and effort with improved high-content screening
What is EurekaScan™ Finder?
- Thermo Scientific EurekaScan™ Finder is a new feature for HCS Studio software that significantly reduces your instrument runtime (CellInsight CX7 LED and CellInsight CX7 LZR) and analysis efforts by automating the detection and subsequent capture of events at higher magnification.
Why is there a need for EurekaScan™ for high-content screening?
- Without EurekaScan™ Finder, if the well is sparsely populated or if you need to image rare events at high magnification, scan times are significantly enhanced due to the instrument performing autofocus and imaging on the blank fields.
- Also, typical analysis times are prolonged as you need to review and discard a large number of irrelevant images.
The solution is EurekaScan™ Finder
- With EurekaScan™ Finder objects/events recognized at lower magnification during the first scan are automatically used to drive the scan area of the high magnification pass(es), thus saving time and effort.
- EurekaScan™ Finder can also handle more than two passes (double-pass high-content screening) for those scenarios that could benefit from three or more passes, such as identifying non-sparse fields at low magnification, finding rare events at higher magnification, and then evaluation of rare events at same or higher magnification.
Click image to enlarge
Figure 2. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X screen alone without EurekaScan™ Feature. All images acquired and analyzed. Data provided from Dr. Chi Yun at New York University.
Figure 2. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X screen alone without EurekaScan™ Feature. All images acquired and analyzed. Data provided from Dr. Chi Yun at New York University.
Click image to enlarge
Figure 3. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X EurekaScan™ screen. The EurekaScan™ Feature auto-selects the area of interest for imaging.
*EurekaScan™ Finder utilization produced a 14.6-fold improvement in screening time compared to conventional Non-EurekaScan Finder 10X alone. Additional efficiencies were captured when evaluating the file size of the two screens, with EurekaScan™ Feature exhibiting 15-fold improvement in memory usage to complete the screen. Data provided from Dr. Chi Yun at New York University.
Figure 3. Human IPS spheroids were fluorescently labeled with DAPI nuclear counterstain and then screened on the CellInsight CX7 LZR platform with 10X EurekaScan™ screen. The EurekaScan™ Feature auto-selects the area of interest for imaging.
*EurekaScan™ Finder utilization produced a 14.6-fold improvement in screening time compared to conventional Non-EurekaScan Finder 10X alone. Additional efficiencies were captured when evaluating the file size of the two screens, with EurekaScan™ Feature exhibiting 15-fold improvement in memory usage to complete the screen. Data provided from Dr. Chi Yun at New York University.
Click image to enlarge
Figure 4. HCS Studio EurekaScan™ Feature results. During the 10X screen, both widefield and 70-micron pinhole confocal images were acquired using the 405 lazer. Data provided from Dr. Chi Yun at New York University.
Flexible with various sample types
EurekaScan™ Finder offers two different approaches based on the type of sample and user-stipulated criteria that will inform high-content screening:
Scenario I: when studying tissues
Click image to enlarge
Scenario I: In this scenario (tissue), the selected objects in first pass are bigger than the second pass’s field of view (case A), or multiple objects appear in the second pass’s field of view (case B).
In which case, the second pass will utilize a uniformly spaced lattice pattern to define the second pass’s field locations.
Scenario II: when studying spheroids, rare-events
Click image to enlarge
Scenario II: In this scenario (spheroids, rare events), the selected objects in the first pass are smaller than the second pass’s field of view and are spaced apart such that multiple objects are unlikely to be in the same field of view.
In which case, the second pass will center the field of views around each object.
Note: The software will automatically choose whether to “center-on-object” or use “lattice pattern” based upon each the selected objects average size and density, on a per-well basis.
Smart selection for high-content imaging and screening
- With the EurekaScan™ Feature applied, specimens including spheroids and tissues may be identified during “seek” operations at low magnification and once “found,” efficiently scanned at higher magnifications for optimal resolution.
- The EurekaScan™ Feature was designed to enable multiple pass scans, such as identifying samples at low magnification across large surface areas, capturing samples at intermediate magnification, and evaluating for rare events or improved resolution at higher magnifications.
- By employing the EurekaScan™ Feature for high-magnification scans, you can experience efficiencies in total scan times and corresponding file memory consumption.
- Moreover, object identification and verification parameters are applied in real time during EurekaScan™ Feature operation, so scientists have more confidence that the samples being adaptively examined are relevant to their research.
- Each pass can have its own unique analysis and identification process based on user-stipulated criteria. This includes different variables such as objectives, channels, different z-stacks, or even kinetics.
- Further acquisition time can be shortened by locking the same the reference position used for the z-stack acquisition during first pass and then using it for subsequent passes.
Click image to enlarge
Figure 5. A 4X scan of a single well of a 24-well plate containing brain organoids stained with tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) was performed. Brightfield was used for object selection for the second scan. The second pass is 20X, brightfield, widefield (WF) tyrosine hydroxylase (Alexa Fluor 488), WF neurofilament (Alexa Fluor 568) and confocal (CF) tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) performed using CellInsight CX7 LZR high content.
Figure 5. A 4X scan of a single well of a 24-well plate containing brain organoids stained with tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) was performed. Brightfield was used for object selection for the second scan. The second pass is 20X, brightfield, widefield (WF) tyrosine hydroxylase (Alexa Fluor 488), WF neurofilament (Alexa Fluor 568) and confocal (CF) tyrosine hydroxylase (Alexa Fluor 488) and neurofilament (Alexa Fluor 568) performed using CellInsight CX7 LZR high content.
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